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目的探讨全氟辛烷磺酸(PFOS)与大鼠中枢神经系统兴奋性氨基酸和谷氨酰胺合成酶(GS酶)的剂量反应关系。方法将20只成年雄性Wister大鼠随机分为4组(对照组、4个染毒组)每组5只。大鼠染毒PFOS(12.5、25、50mg/kg)5天后,对照组给予等体积的2%吐温-80溶液,应用高效液相色谱法测定脑组织氨基酸类神经递质及谷氨酰胺合成酶的改变。结果与对照组比较,大鼠自发活动能力下降;50mg/kg体重组海马谷氨酸含量显著降低,25和50mg/kg体重组皮质谷氨酸含量显著降低;随着染毒剂量的增加海马GS酶活性增加,其中12.5mg/kg剂量组和25mg/kg剂量组明显高于对照组(P<0.05)。结论氨基酸类神经递质含量的改变是PFOS神经毒性的机制之一。
Objective To investigate the dose-response relationship between perfluorooctane sulfonate (PFOS) and excitatory amino acids and glutamine synthetase (GSase) in the central nervous system of rats. Methods Twenty adult male Wister rats were randomly divided into 4 groups (control group, 4 exposure groups) with 5 mice in each group. Five days after exposure to PFOS (12.5, 25 and 50 mg / kg), rats in the control group were given an equal volume of 2% Tween-80 solution. Amino acid neurotransmitters and glutamine synthesis in brain tissue were determined by high performance liquid chromatography Changes in enzymes. Results Compared with the control group, the spontaneous activity of the rats decreased. The content of glutamate in the hippocampus of 50 mg / kg body weight group decreased significantly, while the content of glutamate in 25 and 50 mg / kg body weight group decreased significantly. Enzyme activity increased, of which 12.5mg / kg dose group and 25mg / kg dose group was significantly higher than the control group (P <0.05). Conclusion The change of amino acid neurotransmitters is one of the mechanisms of neurotoxicity of PFOS.