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本研究旨在为延髓面神经后核内侧区(medial region of nucleus retrofacialis,mNRF)是基本节律性呼吸发生部位的学说提供直接的实验依据。仿Suzue方法制作新生大鼠含有舌下神经根及mNRF的离体延髓脑片标本,以吸附电极记录舌下神经根呼吸节律性放电活动(respiratory rhythmic discharge activity,RRDA)作为呼吸活动的指标,采用全细胞膜片钳记录模式在mNRF同步记录呼吸神经元电活动。观察呼吸非起步神经元、起步神经元的电生理学特征及后者对Cd2+敏感性。在mNRF,呼吸起步神经元放电具有自发性、节律性及电压依赖性发放反应的特征,给予去极化/超极化电流时会产生异位发放。起步、非起步呼吸神经元的各种电学特性(膜电容、输入电阻、内向漏电流)之间无显著性差异。在Sprague-Dawley新生大鼠,呼吸中枢以Cd2+非敏感性呼吸起步神经元为主。本研究进一步表明,mNRF是基本节律性呼吸发生的部位。
The purpose of this study is to provide a direct experimental basis for the hypothesis that the medial region of nucleus retrofacialis (mNRF) is a site of basic rhythmic respiration. The neonatal rat sublingual nerve root and mNRF isolated medulla oblongata slice samples were made by imitating Suzue method. Respiratory rhythmic discharge activity (RRDA) was recorded as the index of respiration activity by the adsorption electrode. Whole-cell patch-clamp recording mode synchronously records respiratory electrical activity in mNRF. To observe the electrophysiological characteristics of non-initiating respiratory neurons and starting neurons and their sensitivity to Cd2 +. In mNRF, spontaneous, rhythmic, and voltage-dependent discharges of respiratory initiating neurons are characteristic of discharge, which can produce ectopic delivery when depolarized / hyperpolarised. There was no significant difference between the various electrical characteristics of starting and non-initiating respiratory neurons (membrane capacitance, input resistance, internal leakage current). In Sprague-Dawley neonatal rats, the respiratory centers predominately initiate Cd2 + non-sensitive respiration neurons. This study further shows that mNRF is the site of basic rhythmic respiration.