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合成了苦味酸铕与N,N-二苄基-N′-苯基-1,1′-联萘-2,2′-二(氧杂乙酰胺)(L)的稀土配合物。经元素分析,IR,TG-DTA和摩尔电导率的表征,确定其组成为Eu(pic)3L.2H2O。测定了配合物在不同溶剂中的荧光强度,发现其荧光强度随溶剂配位能力增强而减弱。通过紫外吸收光谱、荧光发射光谱、粘度法,稳态荧光猝灭方法及其与溴化乙啶(EB)的竞争实验研究了配合物与ct-DNA的作用情况,结果显示,配合物与ct-DNA作用时,其紫外吸收产生明显增色效应,荧光强度增强;EB-DNA体系的荧光强度随配合物的加入迅速减弱;在ct-DNA存在下,配合物被猝灭剂K4[Fe(CN)6]的发光猝灭程度减小;配合物的加入使ct-DNA的粘度增加;实验结果证明,配合物与ct-DNA以插入方式结合,其结合常数Kq=2.665×104L.mol-1。
A rare earth complex of europium picrate and N, N-dibenzyl-N’-phenyl-1,1’-binaphthyl-2,2’-bis (oxaacetamide) (L) was synthesized. The elemental analysis, IR, TG-DTA and molar conductivity were characterized to determine the composition of Eu (pic) 3L.2H2O. The fluorescence intensities of the complexes in different solvents were determined and the fluorescence intensity of the complexes was found to be weakened with the increase of the solvent coordination ability. The effect of the complex with ct-DNA was studied by UV absorption spectroscopy, fluorescence emission spectroscopy, viscometry, steady-state fluorescence quenching and its competition with ethidium bromide (EB). The results showed that the complex with ct The fluorescence intensity of EB-DNA system was rapidly decreased with the addition of the complex; In the presence of ct-DNA, the complex was quenched by the quencher K4 [Fe (CN ) 6] decreased; the addition of the complex increased the viscosity of ct-DNA; the experimental results show that the combination with the ct-DNA by insertion, the binding constant Kq = 2.665 × 104L.mol-1 .