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目的探讨高功率毫米波辐射不同时间对小鼠皮肤的损伤效应。方法选择120只雄性昆明种小鼠,采用20 W/cm294 GHz的发射装置进行照射,按辐射时间随机分为50 s、100 s、150 s组和对照组4个组,每组又分为剪毛(5只)和未剪毛两个小组(25只),辐射后即刻采用红外测温仪监测体温,分别于辐射后即刻、12 h、7 d、14 d和28 d观察小鼠照射局部体表变化,之后每组活杀5只,取辐射局部皮肤,进行HE和天狼星红染色。观察辐射后不同时间皮肤的形态结构改变。结果温度测定结果显示,辐射过程中小鼠体温呈进行性升高,各辐射组分别在照射50 s、100 s和150 s时体表温度升高到峰值,温度升高程度与辐射时间呈正相关。辐射50 s组小鼠真皮血管扩张、水肿;100 s组表皮角质层断裂、脱落,真皮水肿,胶原纤维减少;辐射150 s组辐射后12 h,表皮角化层断裂、解离或脱落,真皮层出血水肿,纤维组织稀疏,毛囊上皮不同程度变性和萎缩;辐射后7 d真皮层胶原纤维密度大,肌层萎缩;辐射后28 d真皮层毛囊功能活跃,纤维组织密集。胶原纤维特染显示,50 s组胶原纤维较为密集;100 s组胶原纤维较稀疏,略肿胀,Ⅲ型胶原增多;150 s组胶原纤维稀疏。结论 94 GHz高功率毫米波辐射50~150 s均可引起小鼠体温升高及表皮和真皮等组织结构的损伤,且上述改变与辐射时间呈正相关。
Objective To investigate the effect of high power millimeter wave radiation on mouse skin damage at different times. Methods A total of 120 male Kunming mice were selected and irradiated by a 20 W / cm294 GHz launcher. They were randomly divided into four groups according to the irradiation time: 50 s, 100 s, 150 s and control group. Each group was divided into two groups: (5) and no shearing (25). The body temperature was monitored by infrared thermometer immediately after irradiation. The mice were exposed to local body surface immediately after irradiation, 12 h, 7 d, 14 d and 28 d After the change, after each group killed 5, take the radiation of the local skin, HE and Sirius red staining. Observation of the morphological changes of the skin at different times after irradiation. Results The results of temperature measurement showed that the body temperature of mice increased progressively during radiation. The body temperature increased to the peak value at 50 s, 100 s and 150 s in each radiation group. The temperature rise was positively correlated with the radiation time. At 50 s irradiation, the dermal vasodilation and edema in the mice of the 50 s group were severed, the stratum corneum was broken, detached, dermal edema and collagen fibers decreased in the 100 s group. The epidermis keratinized layer was cracked, dissociated or exfoliated, Hemorrhagic edema, fibrous tissue sparse, varying degrees of hair follicle epithelial degeneration and atrophy; 7 d after irradiation dermal collagen density, muscle atrophy; 28 days after irradiation dermal layer hair follicle function is active, fibrous tissue dense. The special collagen fibers showed that 50 s collagen fibers were more dense; 100 s collagen fibers more sparse, slightly swollen, type Ⅲ collagen increased; 150 s collagen fibers sparse. Conclusion High-power millimeter-wave irradiation at 94 GHz for 50-150 s can induce the rise of body temperature and the damage of the epidermis and dermis, and the above changes are positively correlated with the irradiation time.