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目的:探讨通管丸对炎性阻塞性不孕大鼠输卵管上皮组织中TNF-α含量的影响。方法:将84只SPF级wistar雌性大鼠随机分为正常组12只、模型组60只、假手术组12只,用混合菌接种法注入输卵管复制炎性阻塞性模型,造模成功后的大鼠随机分为通管丸高、中、低剂量组,模型对照组及妇科千金胶囊组,每组各10只。造模20d后,每组用相应的药物连续灌胃20d,第21天处死、取材,采用HE染色法观察输卵管组织形态学改变,采用免疫组化法检测输卵管上皮组织中TNF-α含量。结果:模型对照组输卵管上皮细胞TNF-α阳性表达明显高于其他各组,差异具有统计学意义(P<0.05);通管丸高剂量组TNF-α阳性表达明显低于中、低剂量组及妇科千金胶囊组,差异具有统计学意义(P<0.05);中、低剂量组与妇科千金胶囊组无明显差异(P>0.05)。结论:通管丸能够调节输卵管上皮细胞TNF-α的表达,达到消除炎症的目的,是治疗输卵管炎性阻塞性不孕的有效丸剂。
Objective: To investigate the effect of Tong Guan Wan on the content of TNF-α in oviduct epithelium of rats with inflammatory obstructive sterility. Methods: Eighty - four SPF wistar female rats were randomly divided into normal group (n = 12), model group (n = 60) and sham operation group (n = 12). The mixed spores were injected into the oviduct to duplicate the inflammatory obstructive model. Rats were randomly divided into Tong Guan Wan high, medium and low dose groups, model control group and gynecological Qianjin capsule group, each group of 10. After modeling for 20 days, the rats in each group were given gavage for 20 days continuously. The animals were sacrificed on the 21st day. The morphological changes of the fallopian tubes were observed by HE staining. The contents of TNF-α in the tubal epithelial tissues were detected by immunohistochemistry. Results: The positive expression of TNF-α in oviduct epithelial cells in model control group was significantly higher than that in other groups (P <0.05). The positive expression of TNF-α in high dose group of Tong Guan Wan was significantly lower than that in middle and low dose group And Gynecological Qianjin capsule group, the difference was statistically significant (P <0.05); middle and low dose group and gynecological Qianjin capsule group had no significant difference (P> 0.05). Conclusion: Tong Guan Wan can regulate the expression of TNF-α in fallopian tube epithelial cells and achieve the goal of eliminating inflammation. It is an effective pill to treat tubal obstructive infertility.