本文介绍一种在保留胞浆的培养淋巴细胞中进行微核分析的新方法。将此方法的结果和经低渗处理淋巴细胞所得结果进行了比较。并且对影响微核频率变异的因素及其细胞学困难,培养时间,方法差异,观察者间的差异,培养技术间的区别,分析所需的时间以及与检测诱变活性的其他方法间的关系等问题进行了讨论。作者使用的方法如下: This article describes a new method for micronucleus analysis in cultured cytoplasmic cultured lymphocytes. The results of this method were compared with the results obtained from the hypotonic treatment of lymphocytes. And the factors affecting the frequency of micronuclei and their cytological difficulties, culture time, method differences, differences between observers, differences in culture techniques, the time required for analysis and the relationship with other methods of detecting mutagenic activity And other issues were discussed. The author uses the following method: