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目的观察无机砷对人皮肤成纤维细胞的DNA损伤效应。方法用0.5、1.0和5.0μmol/L亚砷酸钠诱导离体培养人皮肤成纤维细胞,用彗星实验观察DNA损伤效应。结果对照组的拖尾细胞率和拖尾细胞尾长分别为19.0%和(28.84±10.10)μm。0.5、1.0和5.0μmol/L亚砷酸钠剂量组的拖尾细胞率分别为21.0%、31.0%和41.0%,3个染毒组间的差异有统计学意义(P<0.01);3个染毒组的拖尾细胞尾长分别为(28.05±7.71)、(58.13±10.98)和(72.78±11.03)μm,其差异也有统计学意义(P<0.01)。1.0、5.0μmol/L剂量组的细胞尾长均高于对照组,差异均有统计学意义(P<0.01)。当浓度升至10.0μmol/L时,亚砷酸钠呈现出明显的细胞毒性,存活率仅为64.3%。结论亚砷酸钠对人皮肤成纤维细胞的遗传损伤作用存在阈值范围。浓度为1.0μmol/L时,呈现明显的遗传毒性,且随剂量增加,遗传损伤效应增强。但10.0μmol/L的亚砷酸钠以细胞毒性为主。
Objective To observe the DNA damage effect of inorganic arsenic on human dermal fibroblasts. Methods Human skin fibroblasts were induced in vitro with 0.5, 1.0 and 5.0 μmol / L sodium arsenite. DNA damage effects were observed by comet assay. Results In the control group, the tailing cell ratio and the tail length of trailing cells were 19.0% and 28.84 ± 10.10 μm, respectively. Tail cell rates in the groups of 0.5, 1.0 and 5.0 μmol / L sodium arsenite were 21.0%, 31.0% and 41.0%, respectively. There were significant differences among the three exposure groups (P <0.01) The tail lengths of trailing tail cells in the treated group were (28.05 ± 7.71), (58.13 ± 10.98) and (72.78 ± 11.03) μm, respectively, with significant differences (P <0.01). 1.0, 5.0μmol / L dose tail cell length were higher than the control group, the difference was statistically significant (P <0.01). When the concentration rose to 10.0μmol / L, sodium arsenite showed obvious cytotoxicity, the survival rate was only 64.3%. Conclusion There is a threshold range of genetic damage effect of sodium arsenite on human skin fibroblasts. At a concentration of 1.0 μmol / L, it showed obvious genotoxicity, and the effect of genetic damage increased with dose increasing. However, 10.0μmol / L sodium arsenite mainly cytotoxic.