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目的:研究复康片预防卡马西平(CBZ)肝毒性的效果。方法:将48只大鼠随机分为正常对照组、模型对照组、复康片低剂量组、复康片高剂量组共4组,每组12只。正常对照组正常喂养,每d灌胃蒸馏水,其余不做任何处理。余3组建立CBZ致消化系统损伤模型,造模同时复康片低、高剂量组分别按750mg/(kg·d)和1500mg/(kg·d)灌胃复康片1次/d。90d后,脊髓脱臼处死大鼠,腔静脉取血,离心取血清测肝功能,包括天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP),白蛋白(ALB),取肝组织在光镜与电镜下观察。结果:与正常对照组比较,模型对照组肝功能异常,线粒体面积、脂肪滴面积增大,线粒体密度降低,肝脏病理为受损表现。与模型对照组比较,复康片高剂量组大鼠血清AST、ALT、ALP降低,ALB升高;肝细胞核膜结构清晰完整,核周无水肿,粗面内质网结构正常,含有丰富的核糖体,线粒体形态结构正常,线粒体面积与密度、脂肪滴面积恢复(P<0.05)。复康片低剂量组ALT降低,ALB升高;肝细胞线粒体基本正常或偶有轻度水肿,脂肪滴面积较模型对照组减少(P<0.05)。结论:复康片对CBZ所致大鼠肝功能、肝脏超微结构损害有预防性保护作用,以高剂量为优。
OBJECTIVE: To study the effect of Fukang Tablets in preventing hepatotoxicity of carbamazepine (CBZ). Methods: Forty-eight rats were randomly divided into four groups: normal control group, model control group, Fu Kang tablet low-dose group and Fu Kang tablet high-dose group. There were 12 rats in each group. The normal control group was fed normally, distilled water was administered per d, and the rest was not treated. The remaining 3 groups established CBZ-induced digestive system injury model. At the same time, the low-dose and high-dose groups of Fukang Tablets were given 750mg/(kg·d) and 1500mg/(kg·d) gavage for one time/d. After 90 days, the rats were killed by spinal cord dislocation, blood was taken from the vena cava, and the liver function was measured by centrifugation, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Albumin (ALB), taken from liver tissue was observed under light and electron microscopy. RESULTS: Compared with the normal control group, the model group had abnormal liver function, increased mitochondrial area and fat droplet area, decreased mitochondrial density and impaired liver pathology. Compared with the model control group, the serum AST, ALT, and ALP were decreased and the ALB was increased in the high-dose Fukang group rats; the structure of the hepatocyte nuclear membrane was clear and complete, the nucleus was absent, the rough endoplasmic reticulum was normal, and contained rich ribose Body, normal mitochondrial morphology, mitochondrial area and density, lipid droplet area recovery (P <0.05). The low-dose acetaminophen group showed decreased ALT and elevated ALB; the mitochondria of liver cells were normal or occasionally slightly edema, and the area of fat droplets was decreased compared with the model control group (P<0.05). Conclusion: Fukang Tablets has preventive protective effects on liver function and liver ultrastructure damage in rats induced by CBZ, and is superior at high doses.