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本文旨在研究慢性背根节压迫(chronic compression ofdorsal root ganglion,CCD)模型大鼠背根节(dorsal root ganglion,DRG)神经元自发放电活动对脊髓背角广动力(wide dynamic range,WDR)神经元兴奋性的影响。以大鼠为实验对象,随机分为正常对照组和CCD组,在体情况下对DRG和脊髓背角WDR神经元分别进行细胞内和细胞外记录。结果显示,CCD术后,WDR神经元自发放电发生率及放电频率与正常对照组比较均增加(P<0.05),分别为59.46%和(4.30±0.69)Hz。在CCD大鼠DRG局部给予50 nmol/L河豚毒素(tetrodotoxin,TTX),WDR神经元自发放电活动从加药前的(191.97±45.20)/min降至(92.50±30.32)/min,差异有统计学意义(P<0.05);而正常对照组没有自发放电活动的WDR神经元在DRG局部给予100 mmol/L氯化钾后出现高频放电,洗脱后恢复(n=5)。给予坐骨神经非痛电刺激在正常对照组WDR神经元没有记录到后放电,在CCD组可以在36.36%(12/33)WDR神经元记录到后放电,L4、L5 DRG局部表面直接给予TTX(50 nmol/L),后放电频率从加药前的(263±56.5)Hz降至(117±30)Hz,差异有统计学意义(P<0.05)。研究结果提示外周神经损伤后,DRG神经元的异位自发放电活动对脊髓WDR神经元兴奋性改变有重要的作用。
The purpose of this study was to investigate the effects of spontaneous discharge activity of dorsal root ganglion (DRG) neurons in rats with chronic compression of dorsal root ganglion (CCD) on the dynamic range of the spinal cord in the wide dynamic range (WDR) Impact of excitability. The rats were randomly divided into normal control group and CCD group. The DRG and WDR neurons in spinal dorsal horn were recorded intracellularly and extracellularly respectively. The results showed that the incidence of spontaneous discharge and the frequency of discharge of WDR neurons were significantly increased (P <0.05) after CCD surgery compared with the control group (59.46% and 4.30 ± 0.69 Hz, respectively). In the DRG of CCD rats, 50 nmol / L tetrodotoxin (TTX) was administered locally, and the spontaneous discharge activity of WDR neurons decreased from (191.97 ± 45.20) / min to (92.50 ± 30.32) (P <0.05). However, WDR neurons with no spontaneous activity in the normal control group showed high frequency discharge after local administration of 100 mmol / L potassium chloride in DRG and recovered after elution (n = 5). Non-painful electrical stimulation to the sciatic nerve WDR neurons in the normal control group were not recorded after the discharge, in the CCD group can be 36.36% (12/33) WDR neurons recorded after discharge, L4, L5 DRG local surface directly given TTX nmol / L), and the post discharge frequency decreased from (263 ± 56.5) Hz to (117 ± 30) Hz before administration. The difference was statistically significant (P <0.05). The results suggest that DRG neurons may play an important role in excitatory changes of WDR neurons after peripheral nerve injury.