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目的:研究乙醛脱氢酶2(ALDH2)基因多态性和大量乙醇摄取导致的脑、肺和肝组织相关疾病的易感性。方法:利用ALDH2(+/+)和ALDH2(-/-)小鼠,采用乙醇经口投药后对脑、肺和肝组织内的8-OHdGDNA加合物进行测定的方法来评价DNA氧化性损伤的程度。结果:在ALDH2(-/-)的脑组织内8-OHdG浓度,投药组(25.96±5.57)ng/mgDNA比对照组(19.11±2.22)ng/mgDNA明显增加,并且AL-DH2(-/-)的投药组8-OHdG浓度比其他3组均有明显的增加。在ALDH2(-/-)的肺组织内8-OHdG浓度,投药组(24.89±3.99)ng/mgDNA比对照组(19.50±3.17)ng/mgDNA明显增加,ALDH2(-/-)肺组织内的8-OHdG浓度在投药组较其他3组也都有明显增加。ALDH2(-/-)的肝组织内的8-OHdG浓度,投药组(29.77±3.75)ng/mgDNA与对照组(28.54±4.23)ng/mg相比,差异无统计学意义,较ALDH2(+/+)组均有明显增加。结论:乙醇反复投药可以诱发脑组织和肺组织氧化性损伤,在ALDH2(-/-)中这种损伤尤为明显,从而一定程度上可能增加癌症发病的危险度。
OBJECTIVE: To investigate the susceptibility of ALDH2 gene polymorphisms and brain, lung and liver tissue-related diseases due to high ethanol intake. METHODS: Oxidative damage to DNA was assessed using ALDH2 (+ / +) and ALDH2 (- / -) mice by measuring the 8-OHdGDNA adduct in brain, lung and liver after oral administration of ethanol Degree. RESULTS: The concentration of 8-OHdG in brain tissue of ALDH2 (- / -) group was significantly higher than that of the control group (25.96 ± 5.57) ng / mg DNA (19.11 ± 2.22) ng / ) Administration group 8-OHdG concentration than the other three groups have a significant increase. The concentration of 8-OHdG in the lung tissue of ALDH2 (- / -) group was significantly higher than that of the control group (19.50 ± 3.17) ng / mg DNA in the administration group (24.89 ± 3.99) ng / 8-OHdG concentration in the administration group than the other three groups also have significantly increased. The concentration of 8-OHdG in liver tissue of ALDH2 (- / -) group was not significantly different from that of control group (29.77 ± 3.75) ng / mg DNA and control group (28.54 ± 4.23) ng / / +) Group were significantly increased. Conclusion: Repeated administration of ethanol can induce oxidative damage of brain and lung tissue. This damage is especially obvious in ALDH2 (- / -), which may increase the risk of cancer to some extent.