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为观察输血传播病毒ＴＴＶ在各类肝病病人中的感染状况，在日本ＴＴＶＯＲＦ_１保守区设计了两对套式引物，采用巢式聚合酶链反应两次扩增血清 ＴＴＶ ＤＮＡ，并对产物进行分子克隆和基因序列分析。结果显示：在非甲－成型和非庚型肝炎病人、血清ＨＢｓＡｇ阳性的肝炎病人、正常献血员、肝炎肝硬化病人、原发性肝癌病人中，血清ＴＴＶ ＤＮＡ阳性率分别为４３．２％（１６／３７）、２８．８％（１５／５２）、９．３％（４／４３）、５１．９％（１４／２７）、３８．５％（５／１３）、３５．０％（１４／４０）和１７．３％（８／４５）。其中非甲－戊型和非庚型肝炎病人、血清ＨＢｓＡｇ阳性肝炎病人的ＡＬＴ平均为（４７２±２７６）ｕ·Ｌ~（－１）和（３８５±２１８）ｕ·Ｌ~（－１）；肝炎肝硬化病人ＴＴＶ ＤＮＡ阳性率显著高于ＨＢｓＡｇ阳性肝炎病人。提示：ＴＴＶ感染和ＡＬＴ升高存在一定的关系；ＴＴＶ在慢性乙型肝炎向肝硬化发展过程中的作用，应进一步研究。
To observe the infection status of transfusion transmitted virus (TTV) in various kinds of liver disease patients, two pairs of nested primers were designed in Japan TTVORF_1 conserved region. TTV DNA was amplified twice by nested polymerase chain reaction (PCR) and the product was cloned And gene sequence analysis. The results showed that the positive rates of serum TTV DNA in non-A-form and non-G hepatitis patients, serum HBsAg-positive hepatitis patients, normal blood donors, hepatitis cirrhosis patients and primary liver cancer patients were 43.2% 16/37), 28.8% (15/52), 9.3% (4/43), 51.9% (14/27), 38.5% (5/13), 35.0% 14/40) and 17.3% (8/45). The average ALT in patients with non-A-E and non-G hepatitis was (472 ± 276) u · L -1 and (385 ± 218) u · L -1, respectively. The positive rate of TTV DNA in hepatitis cirrhosis patients was significantly higher than that in HBsAg positive hepatitis patients. Tip: TTV infection and elevated ALT there is a certain relationship; TTV in chronic hepatitis B to cirrhosis of the role of development should be further studied.