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蛋白C系统是机体重要的凝血反应调节系统,该文组合生化和免疫化学技术分别从人尿中提纯了血栓调节素和从利凡诺去白蛋白人血浆中纯化了蛋白C、蛋白S和蛋白C抑制物。免疫家兔产生抗血清。用氯胺T法制备了~(125)I-蛋白C和~(125)I-蛋白S,Iodogen法制备了~(125)I-蛋白C抑制物,Bolton-Hunter法制备了~(125)I-血栓调节素。采用平衡法分别建立了上述四种化合物的放射免疫分析。蛋白C、蛋白S、蛋白C抑制物和血栓调节素的分析灵敏度分别
The protein C system is an important coagulation response regulation system in the body that combines biochemical and immunochemical techniques to purify thrombomodulin from human urine and to purify protein C, protein S and protein from human levulinic acid albumin C inhibitor. Immunization of rabbits to produce antisera. ~ (125) I-protein C and ~ (125) I-protein S were prepared by chloramine T method and ~ (125) I-protein C inhibitor was prepared by Iodogen method. I-thrombomodulin. The equilibrium method was used to establish the radioimmunoassay of the above four compounds. Analysis sensitivity of protein C, protein S, protein C inhibitor and thrombomodulin respectively