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目的探讨不同检测方法对婴幼儿丙型肝炎病毒感染诊断结果的影响。方法对66例ELISA法检测抗HCV阳性孕妇所生婴幼儿血清,分别用ELISA法检测抗HCV和HCV-cAg,用荧光定量PCR法检测HCV-RNA,并对结果对比分析。结果 66例婴幼儿血清中,61例抗HCV阳性,阳性率92.4%;9例HCV-cAg阳性,阳性率13.6%;11例HCV-RNA阳性,阳性率16.6%。结论抗HCV检测阳性率过高,且有可能漏诊。建议采用HCV-RNA检测和HCV-cAg综合诊断婴幼儿HCV感染。
Objective To investigate the effect of different test methods on the diagnosis of hepatitis C virus infection in infants and young children. Methods Serum anti-HCV and HCV-cAg were detected in 66 cases of anti-HCV positive pregnant women by 66 ELISA. HCV-RNA was detected by real-time quantitative PCR and the results were compared. Results Among the 66 infants and toddlers, 61 were positive for anti-HCV, with a positive rate of 92.4%. 9 were positive for HCV-cAg and the positive rate was 13.6%. The positive rate of HCV-RNA in 11 cases was 16.6%. Conclusion The positive rate of anti-HCV test is too high, and it is possible to miss diagnosis. HCV-RNA and HCV-cAg are recommended for the diagnosis of HCV in infants and young children.