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采用HPLC-PDA法建立三七酚类成分特征图谱,筛选9个特征峰并通过相对保留时间表征其质量关联性,同时测定对羟基苯甲酸、槲皮素、山柰酚、异鼠李素含量,以对羟基苯甲酸为对照表征酚酸类含量,采用分光光度法测定三七中总酚含量,从而对三七酚类成分含量及其相对比值进行质量表征,然后对三七中各酚类成分含量进行关联分析,从而发现各成分含量的关联性、质量分布规律性,同时将不同批次的药材与经过药效验证的参比药材进行关联度分析,结合关联度分析,筛选出质量优佳三七药材;运用本方法可全面精确的控制和评价三七的质量。
The HPLC-PDA method was used to establish the characteristic map of phenolic compounds, and the nine characteristic peaks were screened. The correlations were characterized by relative retention time, and the content of p-hydroxybenzoic acid, quercetin, kaempferol, isorhamnetin , With p-hydroxybenzoic acid as a control phenolic acids content, the determination of total phenolic content of notoginseng by spectrophotometry, so as to paraquat content and relative ratio of the quality of the characterization, and then the three seven seven phenols Content correlation analysis, in order to find the content of each component relevance, mass distribution of regularity, at the same time, different batches of herbs and pharmacodynamics of the reference drug correlation analysis, combined with the degree of correlation analysis, screening quality Good thirty-seven herbs; the use of this method can be fully and accurately control and evaluate the quality of Panax notoginseng.