AIM:To investigate effects of non-starch polysaccharides(NSP)enzymes on pancreatic and small intestinal digestive enzymeactivities in piglet fed diets containing high amounts of barley.METHODS:Sixty crossbred piglets averaging 13.5 kg wererandomly assigned to two treatment groups with threereplications (pens) based on sex and mass.Each group wasfed on the diet based on barley with or without added NSPenzymes (0.15%) for a 40-d period.At the end of theexperiment the pigs were weighed.Three piglets of eachgroup were chosen and slaughtered.Pancreas,digesta fromthe distal end of the duodenum and jejunal mucosa werecollected for determination.Activities of the digestive enzymestrypsin,chymotrypsin,amylase and lipase were determined inthe small intestinal sections as well as in homogenates ofpancreatic tissue.Maltase,sucrase,lactase and γ-glutamyltranspeptidase (γ-GT)activities were analyzed in jejunal mucosa.RESULTS:Supplementation with NSP enzymes improvedgrowth performance of piglets.It showed that NSP enzymeshad no effect on digestive enzyme activities in pancreas,butdecreased the activities of proteolytic enzyme,trypsin,amylaseand lipase in duodenal contents by 57.56%,76.08%,69.03%and 40.22%(P<0.05) compared with control,and increasedγ-GT activities in jejunal mucosa by 118.75%(P<0.05).CONCLUSION:Supplementation with NSP enzymes in barleybased diets could improve piglets’ growth performance,decrease activities of proteolytic enzyme,trypsin,amylaseand lipase in duodenal contents and increase γ-GT activitiesin jejunal mucosa. AIM: To investigate effects of non-starch polysaccharides (NSP) enzymes on pancreatic and small intestinal digestive enzyme activites in piglet fed diets containing high amounts of barley. METHODS: Sixty crossbred piglets averaging 13.5 kg were randomly assigned to two treatment groups with three reactions (pens) based on sex and mass. Each group wasfed on the diet based on barley with or without added NSPenzymes (0.15%) for a 40-d period. At the end of the experiment the pigs were weighed.Three piglets of eachgroup were chosen and slaughtered. Pancreas, digesta from the distal end of the duodenum and jejunal mucosa were collected for determination. Activities of the digestive enzymestrypsin, chymotrypsin, amylase and lipase were determined inthe small intestinal sections as well as in homogenates of pancreatic tissue. Maltase, sucrase, lactase and gamma-glutamyltranspeptidase (γ-GT) activities were analyzed in jejunal mucosa .RESULTS: Supplementation with NSP enzymes improved growth performance of piglets. It showed that NSP enzymeshad no effect on digestive enzyme activities in pancreas, butdecreased the activities of proteolytic enzyme, trypsin, amylase and lipase in duodenal contents by 57.56%, 76.08%, 69.03% and 40.22% (P <0.05) -GT activities in jejunal mucosa by 118.75% (P <0.05) .CONCLUSION: Supplementation with NSP enzymes in barley-based diets could improve piglets’ growth performance, decrease activities of proteolytic enzyme, trypsin, amylase and lipase in duodenal contents and increase γ-GT activitiesin jejunal mucosa.