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通过关节镜技术采取膝关节滑膜并进行培养，分别于第1、4、7、14、28天收获上清液，测定33例上清液IL－1生物活性，其中类风湿关节炎（RA）11例：测定3O例TNF－α生物活性，RAg例；测定44例PGE2含量，RA11例。结果表明：IL－1增殖指数（GI）、TNF－α杀伤率（KR），RA组明显高于非滑膜炎组（对照组），两组比较差异显著（P＜0．05）；且IL－1与TNF－α高值出现的滑膜培养时间有很好的一致性。RA组使用改变病情药（DMARDs），特别是联合治疗的患者，IL－1及TNF－α活性有被压低的趋势。PGE2含量，RA＋滑膜炎组明显高于骨性关节炎＋非滑膜炎组，两组比较差异显著（P＜0．01）。提示细胞因子IL－1及TNF－α在RA发病机理中占有重要地位。本实验结果或许为类风湿新的治疗途径和战略提供一些线索。
The synovium of knee joint was taken and cultured by arthroscopy technique. The supernatants were harvested on the 1st, 4th, 7th, 14th and 28th days, respectively. The bioactivity of IL-1 in 33 supernatants was determined. Rheumatoid arthritis (RA ) 11 cases: 3O cases of TNF-α biological activity, RAg cases; determination of 44 cases of PGE2 content, RA11 cases. The results showed that IL-1 proliferation index (GI) and TNF-α killing rate (KR) in RA group were significantly higher than those in non-synovitis group (P <0.05) There was a good agreement between IL-1 and synovial culture time when TNF-alpha was high. In RA group, the activity of IL-1 and TNF-α tended to decrease with the use of DMARDs, especially in combination therapy. The content of PGE2 in RA + synovitis group was significantly higher than that in the group of osteoarthritis + non-synovitis group (P <0.01). It is suggested that cytokines IL-1 and TNF-α occupy an important position in the pathogenesis of RA. The results of this experiment may provide some clues for new therapeutic approaches and strategies for rheumatoid arthritis.