论文部分内容阅读
以番茄品种Alpatieva 90 5a和品系Line 1的子叶、下胚轴为外植体诱导愈伤组织进行细胞育种 ,培养基中附加不同浓度的制霉菌素 (Nystatin)用于突变体的筛选 ,结果表明 :两个品种制霉菌素的临界致死浓度 (仅存活 2 %微型愈伤组织时的浓度 )分别为 14 0mg/L和180mg/L。再生植株分别在培养皿内 (试管苗 )和温室等条件下进行了抗晚疫病鉴定 ,其突变体抗病性明显高于对照
The callus of cotyledons and hypocotyls of Cultivars Alpatieva 90 5a and Line 1 were inoculated into cotyledons for cell breeding. Different concentrations of Nystatin were used in the screening of mutants. The results showed that : The critical lethal concentration of the two varieties of nystatin (concentration at survival of only 2% of mini-callus) was 14 0 mg / L and 180 mg / L, respectively. The regenerated plants were identified as resistance to late blight in petri dishes (in vitro) and in greenhouse. The resistance of the regenerated plants was significantly higher than that of the control