目的　探讨端粒酶活性检测在常见恶性肿瘤诊断中的作用。　方法　采用改良的银染端粒重复序列扩增方法 ,对 2 83例恶性肿瘤、6 1例良性肿瘤和 42例癌旁组织进行端粒酶活性检测。结果　 2 83例恶性肿瘤中 ,2 6 4例检测到端粒酶活性 ,阳性率为 93 3 % ;6 1例良性肿瘤中 ,4例检测到端粒酶活性 ,阳性率为 6 6 % ;42例癌旁组织中 ,11例检测到端粒酶活性 ,阳性率为 2 6 2 %。结论端粒酶的激活与恶性肿瘤的发生发展密切相关。端粒酶可以作为一种有效的恶性肿瘤标记物用于恶性肿瘤的诊断。 Objective To investigate the role of detection of telomerase activity in the diagnosis of common malignant tumors. Methods The telomerase activity was detected in 2 83 malignant tumors, 61 benign tumors, and 42 paracancerous tissues using a modified silver-stained telomere repeat sequence amplification method. Results Among 284 malignant tumors, telomerase activity was detected in 264 cases, with a positive rate of 93 3%; in 6 benign tumors, telomerase activity was detected in 4 cases, the positive rate was 66%; 42 In peri-tumor tissues, telomerase activity was detected in 11 cases, with a positive rate of 262%. Conclusion The activation of telomerase is closely related to the occurrence and development of malignant tumors. Telomerase can be used as a marker of malignant tumors for the diagnosis of malignant tumors.