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目的:检测注意缺陷多动障碍患儿与正常儿童的单胺氧化酶B基因表达水平,探计其与注意缺陷多动障碍发病风险的关系。方法:纳入对象为1999-07/09在上海某特殊教育学校中注意缺陷多动障碍患儿40例和江阴市某小学按性别、年龄和种族与患者相匹配原则选择的健康儿童45名。应用定量反转录聚合酶链反应技术检测两组儿童外周血细胞中单胺氧化酶B基因的mRNA表达量,分析单胺氧化酶B基因mRNA平均表达量在两组样本间的差异。结果:按实际完成处理,有36例注意缺陷多动障碍患儿和38名健康儿童的有效样本进入结果分析。注意缺陷多动障碍和健康儿童的单胺氧化酶B基因反转录的表达量分别为(0.018±0.008)和(0.018±0.004)amol/μgcDNA,差异无显著性(t=0.02,df=1,P>0.05)。结论:注意缺陷多动障碍和正常儿童单胺氧化酶B基因的表达无差异,验证了单胺氧化酶B与注意缺陷多动障碍无联系。
OBJECTIVE: To detect the monoamine oxidase B gene expression in children with and / or without attention deficit hyperactivity disorder (ADHD) and to explore its relationship with the risk of attention deficit hyperactivity disorder. Methods: Forty - five healthy children with attention deficit hyperactivity disorder (ADHD) and 45 primary healthy children selected from the primary school of Jiangyin City according to sex, age, race and patient were enrolled in this study from July 1999 to September 09 in Shanghai. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of monoamine oxidase B gene in peripheral blood cells of the two groups. The difference of the average expression of monoamine oxidase B mRNA between two groups of samples was analyzed. Results: According to the actual treatment, effective samples of 36 children with ADHD and 38 healthy children entered the result analysis. The levels of monoamine oxidase B gene reverse transcription in children with attention deficit hyperactivity disorder and healthy children were (0.018 ± 0.008) and (0.018 ± 0.004) amol / μgcDNA respectively, with no significant difference (t = 0.02, df = 0.05). CONCLUSION: There is no difference in monoamine oxidase B gene expression between ADHD and normal children, which confirms that monoamine oxidase B is not associated with attention deficit hyperactivity disorder.