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目的 :检测 p MAPK、cyclinD1和 p5 3蛋白在大肠癌中的表达 ,并探讨其相关性。 方法 :免疫组织化学S P法。结果 :140例大肠癌中 p MAPK、cyclinD1和p5 3蛋白的阳性表达率分别为 92 9% (130 / 140 )、87 1% (12 2 / 140 )和 6 6 4% (93/ 140 ) ;5 0例相应癌旁肠粘膜中阳性表达率分别为 4 0 % (2 / 5 0 )、6 0 % (3/ 5 0 )和 2 0 % (1/ 5 0 )。三者阳性表达率和阳性强度均以大肠癌中为高 (P <0 0 1) ;大肠癌中 p MAPK与cyclinD1表达呈明显正相关 (P <0 0 1) ,而与 p5 3蛋白表达无明显相关性 (P >0 .0 5 )。结论 :MAPK活化可能是cylinD1激活的原因之一 ,并在大肠癌发生过程中可能起重要作用 ;而 p5 3基因突变在大肠癌发生中可能不是通过MAPK信号传递通路起作用的。
Objective : To detect the expression of p MAPK, cyclinD1 and p53 protein in colorectal cancer and to explore their correlation. Methods : Immunohistochemistry SP method. Results: The positive rates of p MAPK, cyclinD1 and p53 in 140 cases of colorectal cancer were 92 9% (130 / 140), 87 1% (12 2 / 140) and 6 6 4% (93 / 140) respectively. The positive expression rates of 50 cases of corresponding paracancerous intestinal mucosa were 40% (2/50%), 60% (3/50), and 20% (1/50) respectively. The positive rate and positive intensity of the three genes were all high in colorectal cancer (P < 0.01). There was a positive correlation between p MAPK and cyclinD1 expression in colorectal carcinoma (P <0 01), but no expression of p53 protein. Significant correlation (P > 0.05). Conclusion : Activation of MAPK may be one of the causes of cylinD1 activation and may play an important role in the development of colorectal cancer. However, p53 gene mutation may not play a role through MAPK signaling pathway in the development of colorectal cancer.