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目的通过实验室技术手段,确认引起暴发流行的病原微生物,并确立病原微生物载体与病人之间的相关性。方法根据现场流行病学调查提示,对二次供水5份、桶装水2份、蓄水池水1份、病人肛拭子7份,粪便1份,呕吐物1份,运用RT-PCR技术筛查确立可疑病原微生物。结果 5份二次供水、1份蓄水池水均检出人星状病毒核酸,7份病人肛拭子中4份阳性,1份粪便和1份呕吐物也均检出人星状病毒核酸,同时排除人杯状病毒、轮状病毒和腺病毒核酸的存在。结论本次急性胃肠炎暴发流行是由星状病毒污染水源引起的。
Objective To confirm the pathogenic microorganisms causing the outbreak and to establish the correlation between pathogenic microorganism carriers and patients through laboratory techniques. Methods According to the field epidemiological investigation, 5 copies of secondary water supply, 2 bottled water, 1 reservoir water, 7 patients with anal swab, 1 stool and 1 vomit were applied to screen by RT-PCR Check establish suspicious pathogenic microorganisms. Results Five secondary water supplies and one reservoir water were all detected as human astrovirus nucleic acid. Four of the seven patients were positive for anal swab, and one stool and one vomit detected human astrovirus nucleic acid, At the same time exclude the human calicivirus, rotavirus and adenovirus nucleic acid. Conclusion The outbreak of acute gastroenteritis is caused by the contamination of water source by astrovirus.