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目的: 研究大豆异黄酮对去卵巢大鼠骨密度以及雌激素活性的影响. 方法: 将10～12月龄的雌性Wistar大鼠随机分为6组:假手术组(SHAM)、切卵巢模型组(OVX)、尼尔雌醇组(OVX-E)、小剂量异黄酮组(L-ISO)、中剂量异黄酮组(M-ISO)、大剂量异黄酮组(H-ISO),每组8只.后5组大鼠被切除双侧卵巢,SHAM组只被切除卵巢附近脂肪组织. L-ISO,M-ISO,H-ISO分别灌胃给予30,60,120 mgkg-1的大豆异黄酮,OVX-E组大鼠灌胃给予0.2 mg*kg-1*wk-1的尼尔雌醇,SHAM与OVX组以等剂量的溶剂灌胃,15 wk 后股动脉放血处死动物,收集血液用于血清碱性磷酸酶活性、血清雌二醇水平、血钙、血磷测定,分离出右侧股骨、第2腰椎用于骨密度测量,测定双侧子宫重量. 结果: 与SHAM组相比,OVX组股骨骨密度和椎骨骨密度均可见不同程度降低(P0.05),尼尔雌醇与异黄酮120mg*kg-1能明显升高去卵巢大鼠的股骨和椎骨骨密度(P<0.05).OVX组的血清碱性磷酸酶水平高于SHAM组(P<0.05),异黄酮60 mg*kg-1可降低去卵巢大鼠的血清碱性磷酸酶水平(P<0.05).OVX组的血清雌二醇水平与子宫系数明显低于SHAM组(P<0.01),异黄酮的不同剂量组与OVX-E组的血清雌二醇水平、子宫系数都明显高于OVX组.虽然异黄酮的不同剂量组的血清雌二醇水平都比OVX-E组高(P<0.01),但它们的子宫系数都明显低于OVX-E组(P<0.01).各组的血钙、血磷值无显著性差异.结论:大豆异黄酮可在去卵巢大鼠体内发挥雌激素样作用,大豆异黄酮120mg*kg-1能提高去卵巢大鼠的骨密度,此作用除与雌激素样作用有关外还可能有其他机制参与.“,”AIM: To study effects of soy isoflavones on estrogen-like activity and bone density in ovariectomized adult rats. METHODS: Forty-eight female Wistar rats (10-12 mo old) were randomly divided into six groups. Ovariectomies were performed in the OVX (ovariectomy), OVX-E(OVX+nilestriol), L-ISO (OVX+soy isoflavones 30 mg*kg-1), M-ISO(OVX+soy isoflavones 60 mg*kg-1) and H-ISO(OVX+soy isoflavones 120 mg*kg-1) groups and animals in the SHAM group were subject to sham operation. Soy isoflavones 30,60,120 mg*kg-1 were given in the L-ISO, M-ISO and H-ISO groups respectively, nilestriol 0.2 mg*kg-1*wk-1 was given in OVX-E group, and the vehicle was given in SHAM and OVX groups. The drugs were all oral administered using a stomach tube. RESULTS: Bone mineral density of right femur was lower in OVX group than those in SHAM, H-ISO and OVX-E groups (P<0.05), there was a tendency of bone density in lumbar vertebra to be lower in OVX group than that in SHAM group. H-ISO and OVX-E rats had higher bone density of lumbar vertebra than that in OVX rats (P<0.05). Serum alkaline phosphates (ALP) activities were higher in OVX group than those in SHAM and M-ISO groups (P<0.05). The OVX group had lower serum estrogen level than those of all other groups. Although uterus relative weights in L-, M- and H-ISO groups were all lower than those in SHAM and OVX-E groups, they were higher than that in the OVX group. CONCLUSION: Soy isoflavones might exert estrogen-like effect in ovariectomized rats. Soy isoflavones 120 mg*kg-1 was effective in preventing bone loss induced by ovariectomy. This result suggests that other mechanisms may involve in this bone protective effect in addition to its estrogenic activity.