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目的:研究细胞因子干扰素-γ(IFN-γ)在肺组织中的表达变化与肺组织病理损伤间的相互关系,为肺结核的防治提供参考。方法:毒性结核分枝杆菌H37Rv尾静脉注射BALB/c小鼠建立肺结核动物模型,分别于感染后0(未感染)、2、4、6、8周末,进行肺组织细菌载荷量计数及组织病理观察,免疫组织化学染色检测IFN-γ、IL-2及TNF-α在肺组织的表达。结果:MTB感染后2周末,IFN-γ、IL-2及TNF-α的阳性细胞百分比较0周时均显著升高,感染后6周末(9.25%)至8周末(5.67%),IFN-γ阳性细胞百分比显著下降(P<0.01)。而该时期TNF-α及IL-2的表达均有下降趋势,但与感染后2周或4周时无明显差异(P>0.05)。感染后8周末,肺组织细菌载荷量(8.43)及肺组织病理分数(17)较感染6周时均显著升高(P<0.01)。结论:MTB感染早期,肺组织IFN-γ、TNF-α及IL-2均呈较高表达,对肺组织具有较强的保护作用。MTB持续感染期,IFN-γ表达量下降明显加剧肺组织损伤。
Objective: To study the relationship between the expression of interferon-γ (IFN-γ) in lung tissue and the pathological damage of lung tissue, and to provide reference for the prevention and treatment of pulmonary tuberculosis. Methods: BALB / c mice were injected into the tail vein of Mycobacterium tuberculosis H37Rv to establish a pulmonary tuberculosis animal model. The bacterial load was counted and histopathological changes were observed at the 0 (no infection), 2, 4, 6 and 8 weeks after infection respectively. The expression of IFN-γ, IL-2 and TNF-α in lung tissue were detected by immunohistochemical staining. Results: The percentages of positive cells of IFN-γ, IL-2 and TNF-α at 2 weeks after MTB infection were significantly higher than those at 0 week. The positive rate of IFN-γ at 6 weeks (9.25% Cell percentage decreased significantly (P <0.01). However, the expression of TNF-α and IL-2 decreased at the same time, but there was no significant difference between 2 and 4 weeks after infection (P> 0.05). At the end of the 8th week after infection, the bacterial load of lung tissue (8.43) and the pathological score of lung tissue (17) were significantly higher than those at 6 weeks after infection (P <0.01). CONCLUSION: In the early stage of MTB infection, the expression of IFN-γ, TNF-α and IL-2 in lung tissue are highly expressed and have a strong protective effect on lung tissue. MTB continued infection, decreased expression of IFN-γ significantly increased lung injury.