论文部分内容阅读
【目的】构建新疆粳稻种质资源的DNA指纹图谱,了解新疆粳稻育成品种的遗传相似性。【方法】利用均匀分布于水稻12条染色体的70对SSR引物,以新疆粳稻育成品种及引进资源为材料,进行遗传多样性分析并构建SSR指纹图谱。【结果】有63对引物具有多态性片段,共检测到388个有效等位基因,平对每对引物6.158 7个等位基因;引物平均多态性频率为0.237 1。89个材料间的遗传相似系数在0.72~0.88,相似性较高,以0.73为标准,可分为5大群。【结论】新疆粳稻育成品种的遗传背景相似性较高、多样性不够丰富,遗传基础相对比较狭窄,加强新的基因资源鉴定利用及育种材料创制。
【Objective】 DNA fingerprinting of Xinjiang germplasm resources was constructed to understand the genetic similarity of japonica rice cultivars in Xinjiang. 【Method】 70 pairs of SSR primers uniformly distributed on 12 chromosomes of rice were used to analyze the genetic diversity of japonica rice cultivars and introduced resources in Xinjiang and SSR fingerprinting was constructed. 【Result】 There were 63 pairs of primers with polymorphic fragments, 388 valid alleles and 6.158 7 alleles per primer pair. The average polymorphism frequency of primers was 0.237 1.89 The genetic similarity coefficients ranged from 0.72 to 0.88 with high similarity to 0.73, which could be divided into five groups. 【Conclusion】 The genetic background of japonica rice cultivars in Xinjiang is similar in genetic background, rich in diversity and relatively narrow in genetic basis, and the identification and utilization of new genetic resources and the creation of breeding materials are strengthened.