论文部分内容阅读
DNA重组技术是将两个或多个单独的DNA片段连接起来产生一个能在特定宿主中自主复制的DNA分子。重组DNA分子最简单的构建方法是将基因片段克隆到质粒或噬菌体等克隆载体上。常用的质粒载体有pBR322、pUC和pGEM等。这些载体含有不同的多克隆位点,适于将含有不同酶切位点的DNA片段连接起来[1]。获得性免疫缺陷综合征(acquired immune defi-
DNA recombination technology involves joining two or more individual pieces of DNA to produce a DNA molecule that autonomously replicates in a particular host. The easiest way to construct a recombinant DNA molecule is to clone the gene fragment into a cloning vector such as a plasmid or phage. Commonly used plasmid vectors pBR322, pUC and pGEM and so on. These vectors contain different multiple cloning sites and are suitable for joining DNA fragments containing different restriction sites [1]. Acquired immune deficiency syndrome (acquired immune defi-