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目的:评价周围神经脉冲射频(PRF)对神经病理性痛大鼠脊髓脑源性神经营养因子(BDNF)表达的影响。方法:健康清洁级雄性SD大鼠220只，8周龄，体重250～280 g，采用随机数字表法分为4组(n n＝55)：假手术组(S组)、神经病理性痛组(NP组)、PRF组和假PRF组(SPRF组)。采用坐骨神经选择性损伤(SNI)法建立大鼠神经病理性痛模型。于SNI后第7天，PRF组进行PRF治疗(爆发2次/s，脉宽20 ms，输出电压45 V，最高温度42 ℃，持续刺激6 min)。于SNI前1 d(Tn 0)、SNI后第1、3、5、7天(Tn 1～4)、PRF后第1、3、5、7、9、11、14天(Tn 6～12)时测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL)。于Tn 0、Tn 4、Tn 9、Tn 12时，分别采用ELISA法和Western blot法检测脊髓BDNF表达水平，于PRF后即刻(Tn 5)、Tn 9、Tn 12时采用免疫荧光法检测脊髓BDNF表达。n 结果:与S组比较，NP组、PRF组和SPRF组SNI后各时点MWT降低和TWL缩短，脊髓BDNF表达上调(n P<0.05)；与NP组比较，PRF组PRF治疗后各时点MWT升高和TWL延长，脊髓BDNF表达下调(n P<0.05)。n 结论:PRF缓解大鼠神经病理性痛的机制可能与下调脊髓BDNF表达有关。“,”Objective:To evaluate the effect of peripheral nerve pulsed radiofrequency (PRF) on the expression of brain-derived neurotrophic factor (BDNF) in the spinal cord of rats with neuropathic pain (NP).Methods:Two hundred and twenty healthy clean-grade male Sprague-Dawley rats, aged 8 weeks, weighing 250-280 g, were divided into 4 groups (n n=55 each) by a random number table method: sham operation group (S group), NP group, PRF group, and sham PRF group (SPRF group). The model of NP was established by using spared nerve injury (SNI) in anesthetized rats. On the 7th day after SNI, PRF (2 bursts/s, pulse width 20 ms, output voltage 45 V, maximum temperature 42 ℃, continuous stimulation 6 min) was performed in PRF group. The mechanical paw withdrawal threshold (MWT) and thermal paw shrinkage latency (TWL) were measured at 1 day before SNI (Tn 0), 1, 3, 5, and 7 days after SNI (Tn 1-4), and 1, 3, 5, 7, 9, 11, and 14 days after PRF (Tn 6-12). The expression of BDNF in the spinal cord was detected by enzyme-linked immunosorbent assay and Western blot at Tn 0, Tn 4, Tn 9, and Tn 12, and the expression of BDNF in the spinal cord was detected by immunofluorescence immediately after PRF (Tn 5) and at Tn 9 and Tn 12.n Results:Compared with S group, the MWT was significantly decreased, TWL was shortened, and the expression of BDNF in the spinal cord was increased at each time point after SNI in NP group, PRF group and SPRF group (n P<0.05). Compared with NP group, the MWT was significantly increased, TWL was prolonged, and the expression of BDNF in the spinal cord was down-regulated at each time point after PRF in NP group, PRF group and SPRF group (n P<0.05).n Conclusion:The mechanism by which PRF alleviates NP may be related to down-regulating BDNF expression in the spinal cord of rats.