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目的探索建立急性CO中毒迟发脑病(DEACMP)大鼠模型的有效方法,为其进一步研究提供基础。方法 72只SD大鼠分为空气组、CO I组、CO Ⅱ组,分别给予腹腔注射空气、纯品CO气体追加注射(CO I组首次注射99.99%CO纯品气体120mL.kg-1后,连续3次按首次剂量的1/2追加注射,间隔为4h;CO Ⅱ组追加注射剂量改为首次剂量的2/3,其余同CO I组)并动态监测动脉血HbCO,3周后用Morris水迷宫逃避潜伏期成绩筛选具有认知功能障碍的大鼠,即为DEACMP大鼠模型。抽取DEACMP组和空气组大鼠制备脑组织病理切片,观察脑组织结构及神经细胞变化。结果 CO Ⅱ组大鼠急性期动脉血HbCO维持在50.07%～60.77%;CO Ⅱ组逃避潜伏期高于CO I组及空气组,DEACMP发生率也高于其它两组;DEACMP组大鼠脑组织病理改变主要为神经元变性坏死,大脑白质广泛脱髓鞘。结论首次注射CO纯品气体(99.99%)120mL.kg-1,追加剂量上调为首剂2/3量的腹腔注射方法更适合制作DEACMP大鼠模型。
Objective To explore an effective method to establish a rat model of delayed-onset encephalopathy (CO) induced by CO and provide the basis for further study. Methods Seventy-two SD rats were divided into air group, COI group and CO Ⅱ group, and were given intraperitoneal injection of air and pure CO gas respectively. After the first injection of 99.99% CO pure gas 120 mL · kg-1 in COⅠ group, 3 times in a row for the first dose of 1/2 additional injection interval of 4h; CO Ⅱ group additional injection dose was changed to the first dose of 2/3, the rest with COI group) and dynamic monitoring of arterial blood HbCO, 3 weeks after Morris Water maze escape latency results screening for cognitive dysfunction in rats, that is DEACMP rat model. The rats in DEACMP group and air group were sacrificed to prepare pathological sections of brain tissue, and the changes of brain tissue and nerve cells were observed. Results The HbCO level of arterial blood in CO Ⅱ group was maintained at 50.07% -60.77% in CO Ⅱ group. The escape latency of CO Ⅱ group was higher than that in CO Ⅰ group and air group. The incidence of DEACMP was also higher in CO Ⅱ group than in other two groups. Altered primarily for neuronal degeneration necrosis, extensive demyelination of white matter. Conclusion The first injection of pure CO gas (99.99%) 120mL.kg-1, additional dose up to 2/3 of the first dose of intraperitoneal injection method is more suitable for the production of DEACMP rat model.