细胞免疫试验(E花环试验,PHA刺激的淋巴细胞转化,混合淋巴细胞培养等)由于影响因素众多,重复性较差,难于对临床研究提供可靠信息。国内虽曾集中多家医学院校对E花环试验等制定出统一的操作流程,但收效不大。究其原因,可能与缺乏统一参考品(淋巴细胞)有关。目前,液氮供应在国内已十分普遍,液氮冻存的淋巴细胞 Cellular immunity test (E-garland test, PHA-stimulated lymphocyte transformation, mixed lymphocyte culture, etc.) is difficult to provide reliable information for clinical research because of many factors and poor repeatability. Although a number of medical schools in China concentrated on the E-rosette test and other procedures to develop a unified operation, but with little success. The reason may be related to the lack of a unified reference (lymphocytes). At present, the supply of liquid nitrogen has been very common in China, liquid nitrogen cryopreserved lymphocytes