An effective broad-spectrum fungicide,azoxystrobin (AZ),has been widely detected in aquatic ecosystems,potentially affecting the growth of aquatic microorganisms.In the present study,the eukaryotic alga Monoraphidlium sp.and the cyanobacterium Pseudanabaena sp.were exposed to AZ for 7 days.Our results showed that 0.2-0.5 mg/L concentrations of AZ slightly inhibited the growth of Monoraphidium sp.but stimulated Pseudanabaena sp.growth.Meanwhile,AZ treatment effectively increased the secretion of total organic carbon (TOC) in the culture media of the two species,and this phenomenon was also found in a freshwater microcosm experiment (containing the natural microbial community).We attempted to assess the effect of AZ on the function of aquatic microbial communities through metabolomic analysis and further explore the potential risks of this compound.The metabonomic profiles of the microcosm indicated that the most varied metabolites after AZ treatment were related to the citrate cycle (TCA),fatty acid biosynthesis and purine metabolism.We thereby inferred that the microbial community increased extracellular secretions by adjusting metabolic pathways,which might be a stress response to reduce AZ toxicity.Our results provide an important theoretical basis for further study of fungicide stress responses in aquatic microcosm microbial communities,as well as a good start for further explorations of AZ detoxification mechanisms,which will be valuable for the evaluation of AZ environmental risk.