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目的 :探讨神经营养因子 NGF、BDNF联合使用时对鼠胚基底前脑胆碱能神经元发育生长的协同作用。方法 :实验分成 NGF、BDNF、NGF +BDNF和对照 4组。采用细胞培养和脑内移植手段及 ACh E组化染色、显微测量和图象分析等技术。检测各组胚基底前脑培养 4天和 8天时的 ACh E阳性神经元数、胞体直径、胞体发出的突起数及最长突起长度以及胚基底前脑移植至脑内 4.5个月时移植区内 ACh E阳性神经元数、细胞面积和纤维密度。结果 :NGF主要有利于 ACh E阳性神经元存活 ,并有轻微促进突起发育生长的作用 ,BDNF促进胞体和突起发育生长的作用较强 ;培养 4天时 ,BDNF+NGF组的指标优于 NGF组 ,但和 BDNF组比较无显著性差异。培养 8天时 ,NGF+BDNF组的指标优于 NGF组或 BDNF组。脑移植区中 ,应用神经营养因子动物 ACh E阳性神经元的形态学指标好于对照组 ,而 NGF+BDNF组的指标又优于 NGF组或 BDNF组。结论 :细胞培养中 BDNF对胚基底前脑胆碱能神经元的作用较 NGF早 ;NGF和 BDNF在胚基底前脑胆碱能神经元早期发育生长中的作用各有侧重 ,两者联合使用在体内、外均可发挥协同作用。
Objective: To investigate the synergistic effect of neurotrophic factor NGF and BDNF on the development and development of cholinergic neurons in rat embryo basal forebrain. Methods: The experiment was divided into NGF, BDNF, NGF + BDNF and control group. Using cell culture and intracerebral transplantation and ACh E staining, microscopic measurement and image analysis techniques. The number of ACh E positive neurons, the diameter of soma, the number of neurites and the length of the longest protrusion in the basal forebrain incubated for 4 and 8 days in embryo were detected, ACh E positive neurons, cell area and fiber density. Results: NGF was mainly beneficial to the survival of ACh E positive neurons and slightly promoted the growth and development of neurites. BDNF enhanced the growth of the somatic cells and neurites. After 4 days of culture, BDNF + But no significant difference compared with BDNF group. When cultured for 8 days, the indexes of NGF + BDNF group were better than NGF group or BDNF group. In the brain transplantation area, morphological indexes of ACh E positive neurons in neurotrophic factor animals were better than those in control group, while those in NGF + BDNF group were superior to those in NGF group or BDNF group. CONCLUSION: The effect of BDNF on embryonic basal forebrain cholinergic neurons is earlier than NGF in cell culture. The roles of NGF and BDNF in the early development and development of embryonic basal forebrain cholinergic neurons are different. Both inside and outside can play a synergistic effect.