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在澳大利亚墨尔本,连续两次雷雨后哮喘患者增加10倍,引起哮喘流行的因子包括空气污染以及霉菌孢子等大气微粒,但上述患者对裸麦花粉敏感。该花粉颗粒直径>35μm,不能进入下呼吸道,为此探讨其引起哮喘的机制。方法采集新鲜裸麦花粉,悬浮于蒸馏水中,轻轻摇晃,使花粉颗粒因渗透破裂,经5μm和0.45μm硝化纤维素筛过滤,行蛋白质印迹(Western-blot)分析,并制作破裂花粉颗粒的扫描电镜照片。于花粉季
In Melbourne, Australia, the number of asthmatic patients increases tenfold after two consecutive thunderstorms, and the factors that cause asthma include airborne particles such as air pollution and mold spores, but these patients are sensitive to rye pollen. The pollen particle diameter> 35μm, can not enter the lower respiratory tract, to explore its mechanism of asthma. Methods Fresh rye pollen was harvested, suspended in distilled water and gently shaken. The pollen grains were infiltrated and ruptured. The pollen grains were filtered through 5μm and 0.45μm nitrocellulose sieves and analyzed by Western-blot. Electron microscope photo. In the pollen season