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目的筛选具有较好抑制金黄色葡萄球菌效果的表皮葡萄球菌,研究通过鼻腔进行表皮葡萄球菌调节观察大鼠对变应原敏感性的效果。方法由鼻腔拭子稀释液分离筛选得到的9株表皮葡萄球菌(Staphylococcus epidermidis),使用上清液抑制法选取抑制金黄色葡萄球菌效果最好的表皮葡萄球菌做体内实验。将Wistar大鼠分为阴性组、模型组、表葡调节组。模型组、表葡调节组使用OVA建立呼吸道炎症模型,使用表皮葡萄球菌DMSA16001进行滴鼻调节2周,同时用PBS进行滴鼻对照。统计抓鼻和喷嚏数,用ELISA进行肺灌洗液(BALF)和外周血IgE的检测。结果 (1)不同株的表皮葡萄球菌均对金黄色葡萄球菌有抑制作用,表皮葡萄球菌DMSA16001对金黄色葡萄球菌的抑制率最高。(2)与模型组相比,表葡调节组的抓鼻数显著下降(t=2.173,P=0.049),喷嚏数有下降趋势,表葡调节组的肺灌洗液和外周血血清的IgE水平均呈现下降趋势。结论对金黄色葡萄球菌有抑制作用的表皮葡萄球菌能使OVA造模的哮喘大鼠对变应原敏感性降低。
Objective To screen Staphylococcus epidermidis which has better inhibitory effect on Staphylococcus aureus and to study the effect of Staphylococcus epidermidis through the nasal cavity on allergen sensitization. Methods Nine strains of Staphylococcus epidermidis were isolated and screened from the nasal swab dilutions, and the best one Staphylococcus epidermidis against Staphylococcus aureus was selected using the supernatant inhibition method. Wistar rats were divided into negative group, model group and epilepsy group. The model group and the tabletagregulation group used the OVA to establish a model of airway inflammation, and intranasal administration of Staphylococcus epidermidis DMSA16001 was performed for 2 weeks while the intranasal control was administered with PBS. Counting nose and sneezing number, using ELISA for lung lavage fluid (BALF) and peripheral blood IgE detection. Results (1) Staphylococcus epidermidis of different strains had inhibitory effect on Staphylococcus aureus, and Staphylococcus epidermidis DMSA16001 had the highest inhibitory rate on Staphylococcus aureus. (2) Compared with the model group, the number of scratching nodules in the control group was significantly decreased (t = 2.173, P = 0.049), and the number of sneezing had a decreasing trend. The IgE in lung lavage fluid and peripheral blood serum The level shows a downward trend. Conclusion Staphylococcus epidermidis, which has an inhibitory effect on Staphylococcus aureus, can reduce the sensitivity of allergens to OVA-induced asthma in rats.