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利比亚皇后草的茎段在MS+BA0.8mg/L+2,4-D0.5mg/L培养基上诱导的愈伤组织,在MS+BA1mg/L+GA3mg/L+IAA0.2mg/L的培养基上分化率达18.8个芽/块,建立了高效分化无性系。把愈伤组织的分化苗在1/2MS+IAA0.2mg/L培养基上培养能获得具有大量气生根的生根苗。生根苗经过炼苗,在河沙或炉灰渣的基质上易移栽成活或扦插成活,成活率可达100%。采用组织培养的方法,可以对利比亚皇后草进行试管苗无性繁殖,达到快速繁殖的目的。
The stems of Queen Libya were induced on MS + BA0.8mg / L + 2,4-D0.5mg / L medium with MS + BA1mg / L + GA3mg / L + IAA0.2mg / L The differentiation rate of medium was 18.8 buds per plant, and a highly efficient clonal clone was established. The callus differentiation seedlings were cultured on 1 / 2MS + IAA0.2mg / L medium to obtain rooting plants with a large number of aerial roots. After rooted seedlings harvested, in the river sand or slag on the substrate easily transplanted alive or cuttings survive, the survival rate of up to 100%. The method of tissue culture can be used for the in vitro propagation of test-tube seedlings of queen beetles in Libya, so as to achieve the purpose of rapid breeding.