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目的:探讨survivin和GRIM-19在前列腺癌组织中的表达及意义。方法:应用免疫组化、RT-PCR和Western印迹法检测survivin和GRIM-19在正常前列腺(NP)组织、良性前列腺增生(BPH)组织和前列腺癌(PCa)组织中的表达情况。结果:免疫组化结果显示survivin在NP、BPH和PCa组织中的表达率分别为6.25%、18.18%和90.62%(P<0.01);GRIM-19的表达率分别为87.50%、81.82%和9.37%(P<0.01)。半定量RT-PCR结果显示,在NP和BPH组织未检测到survivinmRNA表达,而PCa组织可检测到survivinmRNA高表达。Western印迹结果证实,在NP和BPH组织中有微量的survivin蛋白表达;而PCa组织可检测到survivin蛋白高表达;在NP和BPH组织中GRIM-19蛋白表达强阳性,而PCa组织中只有微量表达,两者比较差异均有显著性(P<0.01)。结论:survivin和GRIM-19的表达可能与PCa的发生密切相关。
Objective: To investigate the expression and significance of survivin and GRIM-19 in prostate cancer. Methods: The expressions of survivin and GRIM-19 in normal prostatic (NP), benign prostatic hyperplasia (BPH) and prostatic carcinoma (PCa) tissues were detected by immunohistochemistry, RT-PCR and Western blotting. Results: The immunohistochemical results showed that the expression rates of survivin in NP, BPH and PCa tissues were 6.25%, 18.18% and 90.62% (P <0.01), respectively. The expression rates of GRIM-19 were 87.50%, 81.82% and 9.37 % (P <0.01). Semi-quantitative RT-PCR results showed that survivin mRNA expression was not detected in NP and BPH tissues, but high expression of survivin mRNA was detected in PCa tissues. Western blotting results showed that there was a small amount of survivin protein expression in NP and BPH tissues, and high expression of survivin protein in PCa tissues. GRIM-19 protein expression was strongly positive in NP and BPH tissues, while only slight expression in PCa tissues , The difference between the two was significant (P <0.01). Conclusion: The expression of survivin and GRIM-19 may be closely related to the occurrence of PCa.