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长爪沙鼠线粒体ＤＮＡ经分离提纯，采用ＢｚｍＨＩ，ＢｇｌⅡ，ＥｃｏＲⅠ和＊ＰｓｔⅠ四种内切酶对１３只长爪鼠ｍｔＤＭＡ进行了酶切分析。其中大多数长爪沙鼠酶切图谱相同，用上述四种酶切分别产生１、５、６、和３个片段，我们称之为Ａ型；另３只氏爪沙鼠经ＢｇｌⅡｉ和ＥｃｏＲ酶切后分别产生４个片段，称为Ｂ型。利用λ—ＤＮＡＨｉｎｄⅢ、ΦＸ—ｌ７４ＨＡＥⅢ、λ—ＤＮＡＢｓｔＥⅡ酶切片段作为标准，以琼脂糖凝胶电泳法测出长爪沙鼠ｍｔＤＮＡ内切片段大小，各种酶切片段分子量加起来均为１６．０５ｋｂ。经多次ＢａｍＨⅠ对ｍｔＤＮＡ单酶切，发现只有一个切点，以此切点为零点，分别与其它三种酶组合进行双酶切时，均未改变原来各酶单酶切片段大小，这说明另外三个酶均有一个切点接近零点。我们根据双酶解和不完全酶解片段的大小分析确定了各酶切片段之间的相对位置，绘制了ｍｔＤＮＡ的物理图谱。本次得到的长爪沙鼠的ｍｔＤＮＡ物理图谱是以ＢａｍＨⅠ酶切点为零点，ＰｓｔⅠ酶切片段ｃ→ａ为顺时针方向，由四种内切酶对ｍｔＤＮＡ的水解结果而得到的１５个片段组成，其中大部内切酶切点的相对位置是用双酶解结果来定位的，小部分切点（６个）则是利用不完全酶解结果定位的。１５个位点?
The mitochondrial DNA of Meriones unguiculatus was isolated and purified. The mtDMA of 13 long-clawed mice were digested by BzmHI, BglⅡ, EcoRⅠ and * PstⅠrespectively. Most of the gerbils showed the same digestion pattern. The four digests produced 1, 5, 6, and 3 fragments, respectively, which we named as type A. The other three clawed gerbils were identified by BglIIi and EcoR enzyme After cutting were generated four fragments, known as B type. Using λ-DNAHindⅢ, ΦX-l74HAEⅢ, λ-DNABstEⅡ digestion fragment as the standard, the size of mtDNA mtDNA fragments was determined by agarose gel electrophoresis, and the molecular weights of all the digested fragments were 16.05 kb . After multiple BamHⅠ mtDNA single digestion and found that there is only one point of cleavage point to zero point, respectively, in combination with the other three enzymes double digestion, did not change the size of the original enzyme digested fragments, indicating that The other three enzymes have a tangent point close to zero. According to the double digestion and incomplete digestion fragment size analysis to determine the relative position between the digested fragments, drawn mtDNA physical map. The mtDNA physical map of gerbil obtained from this time is based on the BamH Ⅰ enzyme cleavage point of zero, Pst Ⅰ digestion fragment c → a clockwise direction, four endonuclease mtDNA hydrolysis results obtained 15 fragments The relative position of most of the cleavage sites was located by double digestion, while the small number of cleavage sites (6) were located by incomplete digestion. 15 sites?