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目的研究胃炎Ⅰ号对慢性萎缩性胃炎(CAG)大鼠胃黏膜MMP1和TIMP1 mRNA表达的影响,探讨胃炎Ⅰ号对CAG大鼠胃黏膜损伤的疗效作用机制。方法将SD大鼠随机分为正常对照组和造模组,参照文献制造CAG模型;造模成功后采用胃炎I号煎剂高中低剂量(2.16,1.08,0.54 g.kg-1)及维酶素(0.86 g.kg-1)治疗CAG模型大鼠,实时荧光定量PCR技术检测MMP1及TIMP1 mRNA表达。结果与正常对照组比较,CAG组大鼠胃黏膜MMP1 mRNA表达降低(P<0.05),TIMP1 mRNA表达无明显变化(P>0.05);各治疗组与模型组比较,中剂量胃炎I号组对CAG大鼠胃黏膜病变疗效最佳,可提高MMP1 mRNA表达(P<0.05)。结论 CAG大鼠胃黏膜MMP1 mRNA表达水平下降,TIMP1 mRNA的表达未见明显异常,可能是慢性萎缩性胃炎的病理机制之一;胃炎I号可提高胃黏膜组织的MMP1 mRNA表达,有助于胃黏膜组织的修复,则是其治疗慢性萎缩性胃炎的作用机制之一。
Objective To study the effect of Weiyan I on the expression of MMP1 and TIMP1mRNA in gastric mucosa of chronic atrophic gastritis (CAG) rats and to explore the mechanism of the therapeutic effect of Weiyan I on gastric mucosal lesion in CAG rats. Methods SD rats were randomly divided into normal control group and model group. The CAG model was made according to the literature. After the model was successfully established, the rats were treated with gastritis I decoction high, medium and low dose (2.16,1.08,0.54 g.kg-1) (0.86 g.kg-1) treatment of CAG model rats, real-time fluorescence quantitative PCR detection of MMP1 and TIMP1 mRNA expression. Results Compared with the normal control group, the expression of MMP1 mRNA in gastric mucosa of rats in CAG group was decreased (P <0.05) and the expression of TIMP1 mRNA was not significantly different (P> 0.05). Compared with model group, Gastric mucosal lesions of CAG rats have the best curative effect, which can increase the expression of MMP1 mRNA (P <0.05). Conclusions The expression of MMP1 mRNA in gastric mucosa of CAG rats decreased but the expression of TIMP1 mRNA showed no obvious abnormality. It may be one of the pathological mechanisms of chronic atrophic gastritis. Gastritis I can increase the expression of MMP1 mRNA in gastric mucosa and contribute to gastric Mucosal repair, it is one of the mechanisms of treatment of chronic atrophic gastritis.