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实验分为两个阶段 :A .采用复合因素 (CCl4,胆固醇 ,乙醇 )制备大鼠肝硬化模型。B .用自组中药方剂进行治疗 ,观察肝硬化恢复情况和肝细胞凋亡的变化。结果显示 :①用复合因素制备大鼠肝硬化模型的周期为 5 0d。肝硬化大鼠ALT明显升高 ,为 ( 13 5 3 3± 5 9 77)mol/ (s·L) ;A/G比值倒置为 0 60± 0 12。HE染色镜下可见肝组织正常结构消失 ,形成大小不等的假小叶 ,增生和变性坏死的肝细胞并存 ,并可见散在的核固缩、浓染、体积变小的凋亡细胞。流式细胞术分析可见有AP峰出现 ,其细胞凋亡率为 12 3 0 %。C -myc基因表达量明显高于对照组 ,而bcl- 2基因表达量则低于对照组。②肝硬化的大鼠模型用中药治疗 2 0d后 ,体重增加 ,ALT、A/G比值接近正常。病理检查 ,肝硬化时的假小叶逐渐消失 ,变性坏死的细胞减少。DNA电泳显示仍有“Ladder”带纹 ,流式细胞术分析 ,凋亡率、C -myc基因表达量均低于同期的肝硬化非治疗对照 ,而bcl- 2基因则高于对照
The experiment was divided into two stages: A. The rat liver cirrhosis model was made by using complex factors (CCl4, cholesterol and ethanol). B. using self-Chinese medicine treatment, observe the recovery of cirrhosis and changes in hepatocyte apoptosis. The results showed that: (1) The period of rat liver cirrhosis model was 50 days. ALT was significantly increased in cirrhotic rats as (13 5 3 3 ± 5 9 77) mol / (s · L); the A / G ratio was inverted to 0 60 ± 0 12. Hematoxylin-eosin staining showed that the normal structure of liver tissue disappeared and the pseudolobules with different sizes formed. The hepatocytes with hyperplasia and degeneration and necrosis coexisted, and scattered apoptotic cells with nuclear condensation, staining and volume diminished were observed. Flow cytometry analysis showed that AP peak appeared, the apoptosis rate was 123%. C -myc gene expression was significantly higher than the control group, while the bcl-2 gene expression was lower than the control group. ② cirrhosis rat model treated with Chinese medicine 20d, weight gain, ALT, A / G ratio close to normal. Pathological examination, fake lobules gradually disappear when cirrhosis, degeneration and necrosis of cells decreased. DNA electrophoresis showed that there was still “Ladder” banding, flow cytometry analysis, apoptosis rate, C -myc gene expression were lower than the same period of non-treatment of cirrhosis control, while the bcl-2 gene was higher than the control