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为了明确不同香型茶树(Camellia sinensis)品种鲜叶挥发性物质的组成、含量、β-葡萄糖苷酶活性及其基因差异表达特征,以黄观音、0318E、福云6号、0213-2和C19五个四年生茶树品种(系)为实验材料,使用GC-MS测定其鲜叶挥发性物质的组成及含量,并对β-葡萄糖苷酶活性进行测定,同时克隆β-葡萄糖苷酶基因,分析其在不同品种中的表达差异。结果表明,黄观音和0318E的鲜叶中,萜烯类与衍生物种类及含量都高于普通香型茶树鲜叶(福云6号、0213-2和C19);鲜叶中β-葡萄糖苷酶活性以黄观音(7.8 U·g–1)和0318E(7.3 U·g–1)最高,福云6号和C19次之,0213-2(6.1 U·g–1)最低。β-葡萄糖苷酶基因在黄观音和0318E中表达最强,显著高于C19、福云6号和0213-2,在0213-2中的表达量最低。
In order to clarify the composition, content, β-glucosidase activity and differential gene expression of fresh leaves of different fragrant Camellia sinensis cultivars, Huangguanyin, 0318E, Fuyun 6, 0213-2 and C19 Five four-year-old tea varieties (lines) were used as experimental materials. The composition and content of fresh leaves volatile compounds were determined by GC-MS. The β-glucosidase activity was determined. At the same time, β-glucosidase gene was cloned Its expression difference in different varieties. The results showed that the species and content of terpenes and derivatives in fresh yellow leaves and 0318E were higher than fresh leaves of ordinary fragrant tea trees (Fuyun 6, 0213-2 and C19); β-glucoside The enzyme activity was the highest in Huangguanyin (7.8 U · g-1) and 0318E (7.3 U · g-1), the lowest in Fuyun 6 and C19 and the lowest in 0213-2 (6.1 U · g-1). The expression of β-glucosidase was the highest in Huangguanyin and 0318E, significantly higher than that in C19, Fuyun 6 and 0213-2, and the lowest in 0213-2.