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目的:观察黄体酮对切口痛大鼠机械缩足反射阈值（paw mechanical withdraw threshold, PMWT）、脊髓神经激肽-1受体（neurokinin-1 receptor, NK-1R）、血浆P物质表达的影响，探讨黄体酮对疼痛影响的可能机制。方法:采用随机数字表法将24只健康成年雄性Wistar大鼠分为对照组（C组）、黄体酮组（P组，术前3 h肌内注射1.5 mg/100 g）、米非司酮组（M组，术前3 h灌胃1.5 mg/100 g），每组8只。按照Brennan法制备足底切口痛模型，测定大鼠给药前（Tn 0）、手术前（Tn 1）、手术后1 h (Tn 2）、手术后3 h (Tn 3）4个时间点的术侧足PMWT。在Tn 3时间点采用免疫印迹法测定脊髓NK-1R水平，用ELISA法测定血浆P物质水平。n 结果:与C组比较，P组和M组Tn 2、Tn 3时PMWT升高（n P<0.05），且P组PMWT高于M组（n P<0.05）。与C组比较，P组和M组Tn 3时脊髓NK-1R及血浆P物质水平减低（n P<0.05），且P组脊髓NK-1R及血浆P物质水平低于M组（n P<0.05）。n 结论:黄体酮能够抑制切口疼痛，可能与黄体酮受体结合状态抑制脊髓NK-1R的表达及P物质生成和（或）释放有关。“,”Objective:To observe the effects of progesterone on the paw mechanical withdraw threshold (PMWT), spinal cord neurokinin-1 receptor (NK-1R), and plasma substance P expression in rats with incisional pain, and to explore the possible mechanism of progesterone on the pain.Methods:According to the random number table method, 24 healthy adult male Wistar rats were divided into three groups (n n=8): a control group (group C), a progesterone group (group P, intramuscular injection of 1.5 mg/100 g 3 h before operation), and a mifepristone group (group M, intragastric administration of 1.5 mg/100 g 3 h before operation). An paw incisional pain model was established according to Brennan\'s method, and the PMWT of the injured paw was measured at four time points: before administration (Tn 0), before operation (Tn 1), 1 h after operation (Tn 2), and 3 h after operation (Tn 3). Western blot was used to detect the level of NK-1R in the spinal cord, and enzyme-linked immunosorbent assay (ELISA) was used to determine plasma substance P at Tn 3.n Results:Compared with that in group C, PMWT in group P and group M increased at Tn 2 and Tn 3 (n P<0.05), and PMWT in group P was higher than that in group M (n P<0.05). Compared with group C, the levels of spinal cord NK-1R and plasma substance P at Tn 3 in group P and group M decreased (n P<0.05), and the levels of spinal cord NK-1R and plasma substance P in group P were lower than those in group M (n P<0.05).n Conclusions:Progesterone can inhibit incision pain, which may be related to its binding with the progesterone receptor to inhibit the expression of NK-1R and the production and (or) release of substance P.