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化学疗法治疗癌症的主要机制是通过药物调控使无限增殖的癌细胞恢复到正常凋亡,因而实时检测用药过程中癌细胞的凋亡情况对于临床治疗具有重要意义。以卵巢癌SKOV3细胞作为研究对象,针对治疗药物(顺铂)不同浓度培养液的条件下,利用搭建的动态光镊系统在微流控芯片上通过测量捕获效率参数对四组细胞的凋亡情况分别进行检测。实验结果表明,该系统对不同药物浓度培养下SKOV3细胞凋亡情况的检测具有良好效果。与传统的检测手段相比,该系统细胞用量小、耗时短、无需标记、并可实现活体细胞检测,因此为细胞凋亡的临床检测提供了重要指导意义。
Chemotherapy is the main mechanism of cancer treatment through the regulation of drugs to make unlimited proliferation of cancer cells returned to normal apoptosis, and thus real-time detection of cancer cells during the treatment of apoptosis is of clinical significance. To ovarian cancer SKOV3 cells as the research object, for the treatment of drug (cisplatin) different concentrations of culture medium conditions, the use of dynamic optical tweezers system on the microfluidic chip by measuring the efficiency of capture parameters of four groups of cell apoptosis Separate testing. The experimental results show that the system has good effect on the detection of SKOV3 cell apoptosis under different drug concentrations. Compared with the traditional detection methods, the system uses small amount of cells, time-consuming, without labeling, and can achieve the detection of living cells, thus providing an important guide for the clinical detection of apoptosis.