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目的 解决CHO-C28细胞在大规模培养过程中易增厚、脱落和不易维持的难题。方法 试验组用国产改良型DMEM培养基连续培养CHO-C28细胞2个月,对照组用进口和国产DMEM培养基,观察细胞贴壁、增殖、维持和分泌HBsAg情况。结果 试验组细胞贴壁和长满单层时间明显快于对照组,维持2个月细胞未出现增厚和脱落,分泌HBsAg量明显高于对照组。结论 使用国产改良型DMEM培养基培养CHO-C28细胞效果显著优于现用进口和国产DMEM培养基。
Objective To solve the problem that CHO-C28 cells are easy to thicken, fall off and not easy to maintain in large-scale culture. Methods In the experimental group, CHO-C28 cells were continuously cultured in domestic DMEM medium for 2 months. In the control group, imported and domestic DMEM medium was used to observe the adherent, proliferating, maintaining and secreting HBsAg. Results The adherent cells and monolayer cells in the experimental group were significantly faster than those in the control group. The cells in the experimental group did not appear to thicken and fall off after 2 months, and the secretion of HBsAg was significantly higher than that in the control group. CONCLUSION: CHO-C28 cells cultured with modified DMEM in China are significantly better than those of imported DMEM medium and domestic DMEM medium.