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目的探讨二烯丙基三硫醚(DATS)对幽门螺杆菌生物膜的杀菌作用,进一步研究生物膜耐药的机制。方法微量肉汤法测定药物的最小抑菌浓度,平板培养法构建幽门螺杆菌生物膜的体外模型,XTT法检测药物处理后浮游状态与生物膜状态下的幽门螺杆菌活性,激光共聚焦显微镜观察生物膜深层的细菌状态以及活性变化。结果克拉霉素与DATS对浮游状态的幽门螺杆菌均有良好的杀菌作用;使用不同浓度克拉霉素处理后的幽门螺杆菌生物膜活性明显低于对照组;DATS处理后的幽门螺杆菌生物膜活性下降明显,与对照组和克拉霉素组有统计学差异。结论 DATS对浮游状态和生物膜状态下的幽门螺杆菌均具有良好的杀菌活性,可以为耐药菌临床治疗提供参考。
Objective To investigate the bactericidal effect of diallyl trisulfide (DATS) on Helicobacter pylori biofilm and further study the mechanism of biofilm resistance. Methods The minimum inhibitory concentration (MIC) of the drug was determined by micro broth method. The in vitro model of Helicobacter pylori biofilm was constructed by plate culture method. The activity of Helicobacter pylori in the floating state and biofilm after drug treatment was detected by XTT method. Biofilm deep bacterial status and changes in activity. Results Clarithromycin and DATS had a good bactericidal effect on the Helicobacter pylori in the floating state. The activity of H. pylori biofilms treated with different concentrations of clarithromycin was significantly lower than that of the control group. The Helicobacter pylori biofilms treated with DATS Activity decreased significantly, with the control group and clarithromycin group were statistically different. Conclusion DATS has good bactericidal activity against Helicobacter pylori in the state of floating and biofilm, which can provide a reference for the clinical treatment of resistant bacteria.