论文部分内容阅读
Objective:To investigate the effect of insulin-like growth factor-1 (IGF-1) on pituitary tumor transforming gene (PTTG) in glioma C6 cells.Methods:Glioma C6 cells were divided into four groups:A group,treated without IGF-1;B group,treated with 0.1 ng/mL dose of IGF-1;C group,treated with 1 ng/mL dose of IGF-1;D group,treated with 10 ng/mL dose of IGF-1.PTTG mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR),western blotting was used to detect the expression of PTTG protein.Results:The expressions of PTTG mRNA were 1.370±0.212,2.198±0.354,3.452±0.332,and 4.576±0.387 respectively in the four groups,and there was a significantly difference between any two groups (P<0.01).The protein expressions of PTTG in the four groups were 1.407±0.334,1.813±0.465,2.412±0.576,and 3.128±0.665 respectively,and there was a significantly difference between any two groups (P<0.01).Conclusion:IGF-1 can up-regulate the expression of PTTG significantly in dosage-dependent manner.