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目的研究依达拉奉对大鼠缺血脑组织中脑源性神经生长因子(BDNF)和神经生长因子(NGF)表达的影响。方法健康雄性Wistar大鼠,采用改良的Zea-Longa法建立大脑中动脉局灶缺血模型(MCAO)。实验分为模型对照组和依达拉奉组,每组各20只大鼠。依达拉奉组予以腹腔内注射依达拉奉,1次/d,每次3mg/kg;模型对照组每天注射等量生理盐水。72h后行神经功能评分,然后处死,分别取脑梗死周围、海马区和梗死区脑组织作免疫组化染色检测BDNF、NGF蛋白表达变化,原位杂交检测BDNFmRNA、NGFmRNA的表达。结果依达拉奉组神经功能缺损评分低于模型对照组(p<0.05)。依达拉奉治疗组BDNF和NGF蛋白BDNFmRNA和NGFmRNA在梗死区周围和海马区表达均高于模型对照组,差异有统计学意义(p<0.05),在梗死区差异均无统计学意义(p>0.05)。结论依达拉奉可上调大鼠梗死区周围和海马区缺血脑组织中BDNF和NGF的表达,并可能通过此机制起到保护作用。
Objective To investigate the effect of edaravone on the expression of brain-derived nerve growth factor (BDNF) and nerve growth factor (NGF) in ischemic brain tissue of rats. Methods Healthy male Wistar rats were used to establish focal middle cerebral artery occlusion model (MCAO) by the modified Zea-Longa method. The experiment was divided into model control group and edaravone group, 20 rats in each group. Edaravone group were given intradermal injection of edaravone, 1 / d, each 3mg / kg; model control group injected with normal saline. Neurological function scores were performed 72h later, and then sacrificed. The brain tissue around the infarction, hippocampus and infarct area were detected by immunohistochemistry to detect the expression of BDNF and NGF protein. The expression of BDNFmRNA and NGFmRNA were detected by in situ hybridization. Results The edaravone group had lower neurological deficit score than the model control group (p <0.05). The BDNF and NGF mRNA expressions of BDNF and NGF in peripheral blood and hippocampus of edaravone group were significantly higher than those of model control group (p <0.05), and there was no significant difference in infarct area (p > 0.05). Conclusion Edaravone can up-regulate the expression of BDNF and NGF in ischemic brain tissue around the infarct zone and hippocampus in rats, and may play a protective role through this mechanism.