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目的 用碱性单细胞凝胶电泳技术检测苯作业工人外周血淋巴细胞DNA损伤 ,研究苯的遗传毒性。方法 采用碱性单细胞凝胶电泳技术。将接苯工人按累积浓度、历史平均浓度和测定当时 8h时间加权平均浓度 (8hTWA)分组。观察指标包括DNA断裂分级 (将DNA断裂损伤细胞按其损伤程度分级 )及DNA彗星尾长 (彗头末端到彗尾的长度 )。结果 接苯工人DNA断裂程度及DNA彗星尾长 [lg(尾长 +1) ]两个参数 ,接苯组按累积浓度、历史平均浓度和 8hTWA浓度计算 ,均明显高于对照组 ,差异有显著性 (P<0 .0 1) ,并有明显的剂量 -反应关系。结论 彗星试验能够快速、敏感地检测苯引起的人类淋巴细胞DNA损伤 ,提示对于检测环境致癌物和致突变物可能是一有用工具。
Objective To detect the DNA damage of peripheral blood lymphocytes in benzene-exposed workers by alkaline single-cell gel electrophoresis and to study the genotoxicity of benzene. Methods using alkaline single cell gel electrophoresis technology. Will be taken benzene workers according to the cumulative concentration, the historical average concentration and determination of the time 8h time weighted average concentration (8hTWA) grouping. Observations included DNA fragmentation (stratified by the extent of damage to DNA fragmented cells) and DNA tail length (end of comet-tail length). Results The degree of DNA breakage and DNA tail length [lg (tail length +1)] in benzene-exposed workers were significantly higher than those in the control group according to the cumulative concentration, the historical average concentration and the 8hTWA concentration, and the difference was significant (P <0. 01), and there was a significant dose-response relationship. Conclusion Comet assay can detect benzene-induced DNA damage in human lymphocytes rapidly and sensitively, suggesting that it may be a useful tool to detect environmental carcinogens and mutagens.