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以耐盐性较强的砧木1103P为对照品种,对左山一×SO4杂种砧木F1代的6个株系(A15、A17、A34、A35、A38和A48)及左山一×101-1杂种F1代2个株系(B24和B26)的一年生盆栽扦插苗进行100 mmol·L–1 NaCl胁迫处理,以各自无盐胁迫为对照处理。20天后,根据表型计算盐害指数,测定叶绿素含量、光合气体交换参数、叶绿素荧光参数以及生长量指标;以各项生长指标的耐盐系数为耐盐指标,通过主成分分析、相关性分析、隶属函数分析和聚类分析等方法对葡萄株系进行综合评价。结果表明,A34和A35植株无盐害症状,盐害级数为0;A15和A17植株有少部分叶片边缘焦枯,盐害级数为1。盐胁迫大幅度降低了1103P和B26等株系的叶绿素含量、光合速率、新梢生长量和生物量;而A15、A17、A34和A35植株的各项指标降低幅度较小。将生物量等12个单项指标转换成3个相互独立的综合指标,通过聚类分析,发现A34、A35、A15和A17植株的耐盐性较强,A38、A48和B24植株的耐盐性中等,1103P和B26植株的耐盐性较弱,与盐害分级结果一致。
A total of 6 lines (A15, A17, A34, A35, A38 and A48) of F1 generation of Zusanxia × SO4 hybrid stock and Zuanshan × 101-1 hybrid One-year potted cutting seedlings from two F1 lines (B24 and B26) were treated with 100 mmol·L -1 NaCl stress, and their salt-free stress control treatments. After 20 days, the salt damage indices were calculated according to the phenotypes, and the chlorophyll content, photosynthetic gas exchange parameters, chlorophyll fluorescence parameters and growth index were measured. The salt tolerance index of each growth index was the salt tolerance index. The principal component analysis and correlation analysis , Membership function analysis and cluster analysis were used to evaluate grape strains comprehensively. The results showed that the A34 and A35 plants had no salt damage symptoms and the number of salt damage was 0. The leaves of A15 and A17 had a few of edge blight and the number of salt damage was 1. Salt stress significantly reduced the chlorophyll content, photosynthetic rate, shoot growth and biomass of 1103P and B26 lines, while the A15, A17, A34 and A35 plants reduced less significantly. The 12 single indicators such as biomass were converted into three independent comprehensive indicators. Through cluster analysis, it was found that the salt tolerance of A34, A35, A15 and A17 plants was stronger than those of A38, A48 and B24 The salt tolerance of 1103P and B26 plants was weak, which was consistent with the salt damage classification.