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目的探讨开环异落叶松树脂酚二葡萄糖苷(SDG)对雌性去卵巢大鼠心肌缺血再灌注损伤的保护作用。方法雌性3周龄SD大鼠分为7组:假手术组,去卵巢组、再灌注组、SDG干预组(10、20、50、100 mg/kg),生化方法检测大鼠血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL)、低密度脂蛋白胆固醇(LDL)含量,超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,心肌组织中caspase-3比活性;用伊文氏蓝和2,3,5-氯化三苯基四氮唑双染色法测定心肌梗死面积;光镜观察心肌细胞病理变化;用DNA原位末端标记法检测心肌细胞凋亡变化。结果与去卵巢组比较,再灌注组大鼠血清中HDL明显降低(P<0.05),TC、TG、LDL明显升高(P<0.05);心肌病理损伤加重、心肌梗死面积增大;血清SOD水平降低、MDA含量增加(P<0.05);心肌细胞凋亡指数增加(P<0.05);与再灌注组比较,50 mg/kg SDG组大鼠血清中HDL[(3.285±0.327)mmol/L]升高、TC、TG、LDL[分别为(5.508±0.479)、(0.535±0.129)、(3.290±0.358)mmol/L]降低(P<0.05);心肌梗死面积缩小、心肌组织病理损伤减轻;血清SOD水平[(285.786±31.287)U/m L]升高,MDA含量[(2.483±0.391)nmol/m L]降低(P<0.05);心肌细胞凋亡指数(17.57±0.84)明显降低(P<0.05);呈剂量效应关系。结论 SDG对去卵巢大鼠心肌缺血再灌注损伤具有保护作用,其机制可能与SDG提高心肌组织抗氧化能力和减少心肌细胞凋亡有关。
Objective To investigate the protective effect of Larch (LLOX) -phenol diglucoside (SDG) on myocardial ischemia-reperfusion injury in female ovariectomized rats. Methods Female 3-week-old SD rats were divided into 7 groups: Sham operation group, ovariectomized group, reperfusion group and SDG intervention group (10, 20, 50 and 100 mg / kg). Serum total cholesterol TC, TG, HDL, LDL, SOD and MDA, myocardial tissue , And the specific activity of caspase-3 was measured. The area of myocardial infarction was determined by double staining with Evans blue and 2,3,5-triphenyltetrazolium chloride. The pathological changes of cardiomyocytes were observed with light microscope. Cardiomyocyte apoptosis changes. Results Compared with the ovariectomized group, the levels of HDL in the serum of reperfusion group were significantly decreased (P <0.05), TC, TG and LDL were significantly increased (P <0.05); the pathological changes of myocardium were increased and the area of myocardial infarction was increased; (P <0.05), and the apoptosis index of cardiomyocytes increased (P <0.05). Compared with the reperfusion group, the serum HDL level of SDG rats of 50 mg / kg group was significantly higher than that of the reperfusion group [(3.285 ± 0.327) mmol / L ] (P <0.05). The area of myocardial infarction was reduced and the pathological damage of myocardial tissue was alleviated. The levels of TC, TG and LDL [(5.508 ± 0.479), (0.535 ± 0.129) and (3.290 ± 0.358) mmol / (P <0.05). The apoptotic index of cardiomyocytes (17.57 ± 0.84) was significantly lower than that of the control group (P <0.05) (P <0.05); dose-response relationship. Conclusion SDG has a protective effect on myocardial ischemia-reperfusion injury in ovariectomized rats. The mechanism may be related to SDG increasing myocardial antioxidant capacity and reducing cardiomyocyte apoptosis.