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目的探讨1-磷酸鞘氨醇(sphingosine 1-phosphate,S1P)对大鼠血管平滑肌细胞(rat vascular smooth mus-cle cells,rVSMc)迁移的作用机制,为肿瘤血管新生和心血管疾病发生机制的研究提供新的思路。方法体外分离培养、鉴定rVSMc细胞,建立低氧培养箱和氯化钴诱导的细胞低氧模型,应用RT-PCR方法对rVSMc细胞的S1P受体表达水平进行检测,运用微孔隔离室穿越方法研究S1P对rVSMc细胞迁移的作用,并用S1P受体阻断剂加以证实。结果与结论rVSMc细胞表达SPK1和S1P受体rS1P1、rS1P2和rS1P3,低氧状态下rVSMc细胞SPK1的表达和活性均高于对照,S1P主要通过与rS1P2受体结合促进rVSMc细胞的迁移。
OBJECTIVE: To investigate the mechanism of sphingosine 1-phosphate (S1P) on the migration of rat vascular smooth muscle cells (rVSMc) and to investigate the mechanism of tumor angiogenesis and cardiovascular diseases Provide new ideas. Methods The rVSMc cells were isolated and cultured in vitro. The hypoxia model was established by hypoxia incubator and cobalt chloride. The expression of S1P receptor in rVSMc cells was detected by RT-PCR. S1P on rVSMc cell migration and confirmed by S1P receptor blockers. RESULTS AND CONCLUSION: The expression of SPK1 and S1P receptors rS1P1, rS1P2 and rS1P3 in rVSMc cells were higher than those in rVSMc cells. S1P mainly promoted the migration of rVSMc cells by binding to rS1P2 receptor.