论文部分内容阅读
目的研究膀胱癌组织中抑癌基因PTEN和血管内皮生长因子(VEGF)的表达与膀胱癌血管生成的关系。方法应用免疫组织化学S-P法检测62例膀胱癌组织和18例慢性膀胱炎组织(对照组)中PTEN和VEGF的表达,同时分析不同PTEN和VEGF表达状态下,膀胱癌组织微血管密度(MVD)的变化。结果膀胱癌组和对照组PTEN阳性率分别为53.2%(33/62)和100.0%(18/18),2组比较差异有统计学意义(P<0.01);膀胱癌组和对照组VEGF阳性率分别为62.9%(39/62)和27.8%(5/18),2组比较差异有统计学意义(P<0.01);PTEN和VEGF的表达呈负相关(r=-0.832,P<0.01)。将膀胱癌分为4组,组A为PTEN阴性VEGF阳性(n=19),组B为PTEN阴性VEGF阴性(n=10),组C为PTEN阳性VEGF阳性(n=20),组D为PTEN阳性VEGF阴性(n=13)。4组平均MVD分别为41.53、31.40、26.55、25.15,组A显著高于其他3组(P<0.05)。结论膀胱癌PTEN基因失活可能通过增加VEGF的表达来促进血管生成,导致肿瘤恶性进展。
Objective To study the relationship between the expression of tumor suppressor gene PTEN and vascular endothelial growth factor (VEGF) in bladder transitional cell carcinoma and angiogenesis in bladder cancer. Methods Immunohistochemical SP method was used to detect the expression of PTEN and VEGF in 62 cases of bladder cancer and 18 cases of chronic cystitis (control group). The expressions of PTEN and VEGF in different tissues of bladder cancer were analyzed. Variety. Results The positive rates of PTEN in bladder cancer group and control group were 53.2% (33/62) and 100.0% (18/18) respectively, there was significant difference between the two groups (P <0.01). The positive rates of VEGF in bladder cancer group and control group The rates of PTEN and VEGF were negatively correlated (r = -0.832, P <0.01), and the positive rates of PTEN and VEGF were 62.9% (39/62) and 27.8% (5/18) ). The bladder cancer was divided into 4 groups: group A was positive for PTEN negative VEGF (n = 19), group B was negative for PTEN negative VEGF (n = 10), group C was positive for PTEN positive VEGF (n = 20) PTEN positive VEGF negative (n = 13). The mean MVD of the four groups were 41.53, 31.40, 26.55 and 25.15 respectively, and group A was significantly higher than the other three groups (P <0.05). Conclusion Inactivation of PTEN gene in bladder cancer may promote angiogenesis by increasing the expression of VEGF, resulting in malignant progression of the tumor.