论文部分内容阅读
目的 分析抑癌蛋白 (p5 3、p16Ink4、p2 1Waf1、p2 7Kip1)在胰腺癌组织中的异常表达及其与临床和病理特征的关系。方法 采用单克隆抗体及免疫组化Envision法分别检测了 35例胰腺癌、14例壶腹癌和 11例慢性胰腺炎组织中p5 3、p16、p2 1和p2 7的表达情况。结果 p5 3在胰腺癌和慢性胰腺炎的表达阳性率分别为 6 2 9%和 0 (P <0 0 5 )。p16、p2 1和p2 7在胰腺癌的表达缺失率分别为6 0 0 %、37 1%和 6 2 9% ,缺失率明显高于慢性胰腺炎 (P <0 0 5 )。 80 %的胰腺癌组织有 2种以上抑癌蛋白的表达异常。不同抑癌蛋白表达之间无明显相关。除p16表达与肿瘤分化程度相关 (P <0 0 5 )外 ,4种抑癌蛋白表达与年龄、性别、肿瘤大小、部位、淋巴结转移和临床分期等无明显相关。结论 胰腺癌组织中可存在多种抑癌蛋白的表达异常 ,表明抑癌机制的丧失是胰腺癌发生的重要机制
Objective To analyze the abnormal expression of tumor suppressor proteins (p53, p16Ink4, p2 1Waf1, p2 7Kip1) in pancreatic cancer and their relationship with clinical and pathological features. Methods The expression of p53, p16, p21, and p27 in 35 cases of pancreatic cancer, 14 cases of ampullary carcinoma, and 11 cases of chronic pancreatitis were detected by monoclonal antibody and immunohistochemistry method. Results The positive rates of p53 in pancreatic cancer and chronic pancreatitis were 62% and 0 (P < 0.05), respectively. The deletion rates of p16, p21, and p27 in pancreatic cancer were 60%, 371%, and 62%, respectively, and the rate of deletion was significantly higher than that of chronic pancreatitis (P < 0.05). 80% of pancreatic cancer tissues have abnormal expression of two or more tumor suppressor proteins. There was no significant correlation between the expression of different tumor suppressor proteins. Except for the relationship between p16 expression and the degree of tumor differentiation (P < 0.05), the expression of the 4 tumor suppressor proteins was not significantly related to age, gender, tumor size, location, lymph node metastasis, and clinical stage. Conclusion There may be abnormal expression of multiple tumor suppressor proteins in pancreatic cancer tissues, indicating that loss of tumor suppressor mechanism is an important mechanism of pancreatic cancer